Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues
Reexamination Certificate
2006-07-18
2006-07-18
Navarro, Mark (Department: 1645)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
C426S656000, C426S657000
Reexamination Certificate
active
07078488
ABSTRACT:
The present invention relates to a N-glycosylation-modified recombinant chicken cystatin, characterized in that Asn106-Ile108in its amino acid sequence is modified to Asn106-Thr108. The present invention also relates to a method for producing said N-glycosylation-modified recombinant chicken cystatin, wherein a site-directed mutated cDNA encoding chicken cystatin is cloned in an expression vector, with which a yeast strain is in turn transformed, and the yeast transformant is then cultured for producing a recombinant chicken cystatin wherein the Asn residue in the Asn106-Thr108of its amino acid sequence that is produced by said site-directed mutagenesis can be modified by N-glycosylation. The N-glycosylation-modified recombinant chicken cystatin disclosed in the present invention has improved stability in a freezing-thawing process and in a heating process.
REFERENCES:
Shann-Tzong Jiang,Effect of Glycosylation Modification(N-Q-108I→N-O-108T)on the Freezing Stability of Recombinant Chicken Cystatin Overexpressed in Pichia pastoris X-33, J. Agric. Food Chem., 2002, vol. 50, pp. 5313-5317.
Navarro Mark
Nugen Bioscience (Taiwan) Co., Ltd.
Scully , Scott, Murphy & Presser, P.C.
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