Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
2006-12-12
2006-12-12
Qian, Celine (Department: 1636)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C536S023100, C435S252300
Reexamination Certificate
active
07148339
ABSTRACT:
The subject invention provides for a method of selectively cloning homoduplex nucleic acid molecules, in particular, by using a strain of host cells that contains a conditionally expressed and/or conditionally active mismatch-recognizing enzyme, e.g., a temperature sensitive variant of the gene encoding the endonuclease VII from phage T4. Using this host strain, the invention features a novel cloning method that selects for PCR products that are devoid of PCR-generated mutations.
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International Search Report for International Application No. PCT/US03/19575 dated Dec. 1, 2003.
Carstens Carsten Peter
Greener Alan L.
Hexdall Lisa Joy
Sorge Joseph A.
Dunston Jennifer
Edwards Angell Palmer & & Dodge LLP
FitzGerald Mark J.
Qian Celine
Stratagene California
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