Temperature sensitive mutant derivatives of the broad host...

Details Temperature sensitive mutant derivatives of the broad host... Temperature sensitive mutant derivatives of the broad host...

2006-05-16

2006-05-16

Vogel, Nancy (Department: 1636)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

C435S252340, C435S252300, C435S320100, C536S023100

Reexamination Certificate

active

07045338

ABSTRACT:
The present invention describes a mutant plasmid replication control region having the ability to convey temperature sensitivity to the plasmid on which it resides. The mutant replication control region is based on a similar region isolated from pBRH1. Plasmids containing this rggeplication control region cannot be classed as belonging to any known incompatibility group and thus may co-exist with a broad range of other plasmids in a single host.

REFERENCES:
patent: 6365410 (2002-04-01), Schellenberger et al.
patent: 2002/0119573 (2002-08-01), Shaw et al.
patent: 2690459 (1993-10-01), None
Schmidhauser, T. J. et al., Regions of Broad-Host-Range Plasmid RK2 Involved in Replication and Stable Maintenance in Nine Species of Gram-Negative Bacteria, J. Bacteriol. vol. 164: pp. 446-455, 1985.
Szpiner et al.,Escherichia coli. GenBank Y14439. Mar. 10, 2001.
Antoine, R. et al., Isolation and molecular characterization of a novel broad-host-range plasmid from Bordetella bronchlseptica with sequence similarities to plasmids from Gram-positive organisms. Mol. Microbiol. vol. 6(13), pp. 1785-1799, 1992.
Szpirer et al., Interaction between the RP4 coupling protein TraG and the pBHR1 mobilization protein Mob. Molecular Microb, 37(6): pp. 1283-1292, 2000.
Szpirer et al., Mobilization Function of the pBHR1 Plasmid, a Derivative of the Broad-Host-Range Plasmid pBBR1, J. Bacteriol. 183(6): 2101-2110, 2001.

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