Multicellular living organisms and unmodified parts thereof and – Method of making a transgenic nonhuman animal – Via microinjection of a nucleus into an embryo – egg cell – or...
Reexamination Certificate
2007-09-04
2007-09-04
Crouch, Deborah (Department: 1632)
Multicellular living organisms and unmodified parts thereof and
Method of making a transgenic nonhuman animal
Via microinjection of a nucleus into an embryo, egg cell, or...
C435S455000
Reexamination Certificate
active
10105616
ABSTRACT:
This disclosure provides a system for creating cloned cells and embryos that are genetically modified. Cells are treated to increase expression of telomerase and potentially extend replicative capacity. One or more genetic modifications is made to inactivate a gene or confer desirable features, growing and selecting the cells as needed. The modified nucleus can then be transferred to a suitable recipient cell, which can then be used to grow an embryo with the conferred attributes. This technology makes it possible to create embryos, animals and embryonic cell lines with multiple genetic modifications, including homozygously inactivated genes and gene substitutions.
REFERENCES:
patent: 4959317 (1990-09-01), Sauer
patent: 5698763 (1997-12-01), Weissmann et al.
patent: 5821117 (1998-10-01), Sandrin et al.
patent: 5849991 (1998-12-01), d'Apice et al.
patent: 5929301 (1999-07-01), Baszcynski et al.
patent: 6147276 (2000-11-01), Campbell et al.
patent: 6252133 (2001-06-01), Campbell et al.
patent: 6261836 (2001-07-01), Cech et al.
patent: 678234 (1994-11-01), None
patent: WO98/39416 (1998-09-01), None
patent: WO 99/21415 (1999-05-01), None
patent: WO99/21415 (1999-05-01), None
patent: WO99/27113 (1999-06-01), None
patent: WO99/46982 (1999-09-01), None
patent: WO 00/25578 (2000-05-01), None
patent: WO 00/31237 (2000-06-01), None
patent: WO 00/31238 (2000-06-01), None
patent: WO 01/00650 (2001-01-01), None
patent: WO 01/42421 (2001-06-01), None
Simerly, C. et al. Molecular Correlates of Primate Nuclear Transfer Failures. Science. Apr. 11, 2003, vol. 300, p. 297.
Mitalipov et al. Rhesus Monkey Embryos Produced by Nuclear Transfer from Embryonic Blastomeres or Somatic Cells. Biology of Reproduction. 2002, vol. 66, pp. 1367-1373.
Meirelles et al. Complete Replacement of the Mitochondrial Genotype in a Bos indicus Calf Reconstructed by Nuclear Transfer to a Bos taurus Oocyte. Genetics 2001, vol. 158, pp. 351-356.
Fehilly et al. Cytogenic and Blood Group Studies of Sheep/Goat Chimaeras. J. Reproduct. Fertility. 1985, vol. 74, pp. 215-221.
Cibelli et al. Cloned Transgenic Calves Produced from Nonquiescent Fetal Fibroblasts. Science. May 22, 1998, vol. 280, pp. 1256-1258.
Bodnar et al. Extension of Life-Span by Introduction of Telomerase Into Normal Human Cells. Science. Jan. 16, 1998, vol. 279, pp. 349-352.
Yang et al. Human Endothelial Cell Life Extension by Telomerase Expression. Journal of Biological Chemistry. Sep. 10, 1999, vol. 274, pp. 26141-26148.
Denning, C. et al. Gene Targeting in Primary Fetal Fibroblasts from Sheep and Pig. Cloning and Stem Cells. 2001, vol. 3, pp. 221-231.
Cui et al. Telomerase-Immortalized Sheep Fibroblasts Can Be Reprogrammed by Nuclear Transfer to Undergo Early Development. Biol. Reprod. 2003, vol. 69, pp. 15-21.
Bodnar et al., Extension of life-span by introdution of telomerase into normal human cells, Science 279:349 (1998).
Campbell et al., Sheep cloned by nuclear transfer from a cultured cell line, Nature 380:64 (1996).
Clark et al., Gene targeting in livestock: a preview, Transgenic Res. 9:263 (2000).
Clark, Gene expression in the mammary glands of transgenic animals, Biochem. Soc. Symp. 63:133 (1998).
Clark, The mammary gland as a bioreactor: expression, processing, and production of recombinant proteins, Mammary Gland Biol. Neoplasia 3:337 (1998).
Goldmann, et al., Two alleles of a neural protein gene linked to scrapie in sheep, Proc. Natl. Acad. Sci. USA 87:2476 (1990).
Jiang et al., Telomerase expression in human somatic cells does not induce changes associated with a transformed phenotype, Nat. Genet. 21:111 (1999).
Kim et al., Specific association of human telomerase activity with immortal cells and cancer, Science 266:2011 (1997).
Wilmut et al., Viable offspring derived from fetal and adult mammalian cells, Nature 385:810 (1997).
Roslin Signs Six Year Deal With geron, Roslin Biomed Press Release (1999).
Annual Report, Roslin Institute Edinburgh 98-99.
Solter, D., Mammalian cloning: advances and limitations, Nat Rev Genet. 1:199 (2000) Review.
Yang, et al., Human endolethial cell life extension by telomerase expression, J Biol Chem. 274:26141 (1999).
Australian Provisional Patent Application PR 1109, A Novel Cell Type for NuclearTransfer.
Clark A. John
Cui Wei
Denning Chris
Zhao Debbiao
Crouch Deborah
Finnegan Henderson Farabow Garrett & Dunner LLP
Roslin Institute (Edinburgh)
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