Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Patent
1997-05-02
2000-02-15
Brusca, John S.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
435 4, 435194, 435196, 4352542, 435325, 536 231, C12Q 168, C12Q 134, C12N 916
Patent
active
060251352
ABSTRACT:
The strand of telomeric DNA that runs 5' to 3' towards a chromosome end is typically G-rich. Due to the properties of conventional DNA polymerases, telomerase-generated tails of this G-rich strand are expected on only one end of individual linear DNA molecules. In Saccharomyces, TG.sub.1-3 tails are detected on chromosome and linear plasmid telomeres late in S-phase. Moreover, the telomeres of linear plasmids can interact when the TG.sub.1-3 tails are present. Molecules were generated in vitro that mimic the structures predicted for telomere replication intermediates. These in vitro generated molecules formed telomere--telomere interactions similar to those on molecules isolated from yeast but only if both ends that interacted had a TG.sub.1-3 tail. Moreover, TG.sub.1-3 tails were generated in vivo in cells lacking telomerase. These data suggest a new step in telomere maintenance cell cycle regulated degradation of the C.sub.1-3 A strand which can generate a potential substrate for telomerase and telomere binding proteins at every telomere.
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Wellinger et al. Saccharomyces telomeres acquire single-strand TG 1-3 tails late in S phase. Cell vol. 72 pp. 51-60, 1993.
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Wellinger Raymund J.
Zakian Virginia A.
Brusca John S.
The Trustees of Princeton University
Universite de Sherbrooke
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