Techniques for preparing specimens for bacterial assays

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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424 3, 435 4, 435 6, 435 71, 435 34, 435268, 435270, 435810, 435863, 435961, 435962, 435975, C12Q 168, C12S 324, G01N 33569

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053647637

ABSTRACT:
Methods and compositions for improved liquification of mucoid secretion specimens by treatment with a disulfide bond reducing agent and a DNA digestion agent, and for improved concentration of selected bacterial species from such specimens, are disclosed. The disclosure has applicability to bacterial assay techniques including nucleic acid hybridization, culture and stain techniques.

REFERENCES:
patent: 3502779 (1970-03-01), Dye
patent: 3663690 (1972-05-01), Eichel et al.
patent: 4358535 (1982-11-01), Falkow et al.
patent: 4409138 (1983-10-01), Maltz
patent: 4483920 (1984-11-01), Gillespie et al.
patent: 4689294 (1987-08-01), Boguslawski et al.
Lieberman, J., Measurement of Sputum Viscosity in a Cone-Plate Viscometer. II. An Evaluation of Mucolytic Agents in Vitro. American Review of Respiratory Disease 97:662-672 (1968).
Webb, W. R., Clinical Evaluation of a New Mucolytic Agents, Acetyl-Cysteine. Journal of Thoracic and Cardiovascular Surgery 44:330-343 (1962).
Mackay, M., Hilgartner, C. A., and Dounce, A. L., Further Studies of DNA-Nucleoprotein Gels and Residual Protein of Isolated Cell Nuclei. Experimental Cell Research 49:533-557 (1968).
Sherry, S., Tillet, W. S., and Christensen, L. R., Presence and Significance of Desoxyribose Nucleoprotein in the Purulent Pleural Exudates of Patients. Proc. Soc. Exp. Biol. Med. 68: 179-184 (1948).
Tillet, W. S., Sherry, S., and Christensen, L. R., Streptococcal Desoxyribonuclease: Significance in Lysis of Purulent Exudates and Production by Strains of Hemolytic Streptococci. Proc. Soc. Exp. biol. Med. 68: 184-188 (1948).
Lieberman, J. and Kurnick, N. B., Influence of Deoxyribonucleic Acid Content on the Proteolysis of Sputum and Pus. Nature (No. 4859): 988-990 (1962).
Armstrong, J. B., and White, J. C., Liquefaction of Viscous Purulent Exudates by Deoxyribonuclease. Lancet p. 739 (Dec. 9, 1950).
Bornstein, A. A., Chen, T.-M., and Dulfano, M. J., Disulfide Bonds and Sputum Viscoelasticity. Biorheology 15:261-267 (1978).
Puchelle, E., Zahm, J. M., and Havez, R., Biochemical and Rheological Data in Sputum. III-Relationship between the Biochemical Constituents and the Rheological Properties of Sputum. Bull. Physio-Path. Resp. 9: 237-256 (1973) [In French, English abstract cited].
Rawlins, G. A., Liquification of Sputum for Bacteriological Examination. Lancet p. 539 (Sep. 12, 1953).
Lightowler, J. E. and Lightowler, N. M., Comparative Mucolytic Studies on Dithiothreitol, N-Acetyl-cysteine and L-Cysteine on Human Respiratory Mucus In Vitro and Their Effects on the Rate of Flow of Mucus in the Exposed Trachea of the Rat on Topical Application. Arch. Int. Pharmacodyn. 189. 53-58 (1971).
Sherry, S. and Goeller, J. P., The Extent of the Enzymatic Degradation of Desoxyribonucleic Acid (DNA) in Purulent Exudates by Streptodornase. (1950).
Tillett, W. S. and Sherry, S., The Effect in Patients of Streptococcal Fibrinolysin (Streptokinas) and Streptococcal Desoxyribonuclease on Fibrinous, Purulent, and Sanguinous Pleural Exudations. (1948).
Sherry, S. Johnson, A., and Tillett, W. S., The Action of Streptococcal Desoxyribose Nuclease (Streptodornase), In Vitro and on Purulent Pleural Exudations of Patients. (1949).
Kent, Patricia T., and Kubica, George P., Public Health Mycobacteriology; A Guide For The Level III Laboratory. U.S. Department of Health and Human Services, (1985).
Sommers et al, in Lennette et al (Eds.) Manual of Clinical Microbiology, 4th Edition, American Society for Microbiology, Washington, D.C., 1985, pp. 226-229.
Price, P. A., et al., J. Biol. Chem., 244(4):929-932 (1969).
Junowicz, E., et al., Biochim. et Biophys. Acta, 312:72-84 (1973).
Tullis, R. H., et al., Anal. Biochem. 107:26-264 (1980).
Fleisher, et al., Inorg. Chem. 25:3549-3551 (1986).
Hertzberg & Dervan, Biochemistry, 23:3934-3945 (1984).
Amer. Rev. Resp. Disease 97:662-672, 1968 (Lieberman).
Mack & Dervan, J. Am. Chem. Soc. 112:4604-4607 (1990).
Chemical Abstracts 85(23):173830d, 1976 (Martinetti).
Chemical Abstracts 84(19):130190g, 1976 (McNiff, et al.).
Nicolaou, et al., Angew. Chem. Inte. Ed. Engl. 28:1272-1275 (1989).
Sheffner, Ann. N.Y. Acad. Sci., 106:298-310 (1963).
Sigman, Acc. Chem. Res. 19:180-186 (1986).
Sluka, et al., Science 238:1129-1132 (1987).
Taylor, et al., Tetrahedron 40:457-465 (1984).
The Pharmacological Basis of Therapeutics (Goodman & Gilman, eds.), "Mucolytics", pp. 955-956 (5th ed. 1975).
Worthinghton Enzyme Manual, Worthington Biochemical Corporation, Freehold, N.J., 1972, p. 85.

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