Technique for joining amino acid sequences and novel composition

Chemistry: natural resins or derivatives; peptides or proteins; – Peptides of 3 to 100 amino acid residues – Peptides with at least one nonpeptide bond other than a...

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530345, 530402, 530403, 530506, C07K 502

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058408347

ABSTRACT:
Two amino acid sequences are joined together using an electron acceptor moiety and a linking moiety, such as a chelating agent. In particular, an amino acid sequence specific for binding to a material interest is linked to an enzyme which acts on an indicator, such as a colorimetric, phosphometric, fluorometric or chemiluminescent substrate. The linking composition is useful in immunoassays.

REFERENCES:
patent: 4320109 (1982-03-01), Wolf et al.
patent: 5180664 (1993-01-01), Dopatka
patent: 5266686 (1993-11-01), Sorensen
in Immunoassays" Analytical Biochemistry, vol. 229, No. 1 (Jul. 20, 1995), pp. 54-60.

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