Technique for acceleration of apoptotic cell death

Details Technique for acceleration of apoptotic cell death Technique for acceleration of apoptotic cell death

1998-05-22

1999-11-09

Witz, Jean C.

Chemistry: analytical and immunological testing

Biological cellular material tested

436 64, 424531, 514561, G01N 3348

Patent

active

059812842

ABSTRACT:
A first embodiment of a cell culture system has a cell death accelerator comprising one or more cell death inducing substances, including serum albumin, hemoglobin, glycine and glutamic acid. In a second embodiment a cell death inhibitor comprises one or more kinds of cell death inhibiting substances which include mercapto group containing amino acids, other mercapto group containing compounds and tryptophan. In a third embodiment a cell death inhibitor comprises an inhibitor of RNA or protein synthesis, optionally augmented with a thiol. The system can be applied to selectively induce death of cultured cells, such as neoplastic cell lines, or to inhibit death of other cells, such as neoplastic cell lines or non-neoplastic cells such as brain cells.

REFERENCES:
patent: 4533637 (1985-08-01), Yamane et al.
patent: 5248606 (1993-09-01), Walsh et al.
patent: 5272082 (1993-12-01), Santoli et al.
patent: 5298407 (1994-03-01), Anderson et al.
patent: 5360893 (1994-11-01), Owens et al.
patent: 5387520 (1995-02-01), Lopresti et al.
patent: 5468624 (1995-11-01), Thompson et al.
patent: 5476659 (1995-12-01), Goodman et al.
Bissonnette, R.P., F. Echeverri, A Mahboubi, D.R. Green, Apoptotic Cell Death Induced by C-MYC is Inhibited by BCL-2,Nature, vol. 359, Oct. 8, 1992.
Cain, K., S.H. Hussain, L. Kokileva, G.M. Cohen, Multi-Step DNA Cleavage in Rat Liver Nuclei is Inhibited by Thiol Reactive Agents, FEBS Letters, vol. 358, 255-2611 1995.
Cohen, J.J., R.C. Duke, Apoptosis and Programmed Cell Death in Immunity, Annual Review of Immunology, vol. 10, 267-293 1992.
Coles, H.S.R., J.F. Burne, M.C. Raff, Large-Scale Normal Cell Death in the Developing Rat Kidney and its Reduction by Epidermal Growth Factor, Development, vol. 118, 777-784 (1993).
Eastman, A., Highlights Apoptosis: A Product of Programmed and Unprogrammed Cell Death, Toxicology and Applied Pharmacology, vol. 121, 160-164 1993.
Endresen, P.A., P.S. Prytz, S. Lysne, J. Aarbakke, Homocysteine Increases the Relative Number of Apoptotic Cells and Reduces the Relaitve Number of Apoptotic Bodies in HL-60 Cells Treated With 3-Deazaadenosine, The Journal of Pharmacology and Experimental Therapeutics, vol. 269, No. 3, 1245-53 1994.
Endresen, P.C., T.J. Eide, J. Aarbakke, Cell Death Initiated by 3-Deazaadenosine in HL-60 is Apoptosis and is Partially Inhibited by Homocysteine, Biochemical Pharmacology, vol. 46, No. 11, 1893-1901 1993.
Erdoe, Sanor L. "Development of Excitotoxic Vulnerability in Cortical Cultures" Molecular Neuropharmacology vol. 2(3) pp. 245-247 1992.
Evan, G., A Wyllie, C. Gilbert, T. Littlewood, H. Land, M. Brooks, C. Waters, L. Penn, D. Hancock, Induction of Apoptosis in Fibroblasts by C-MYC Protein, Cell, vol. 69, 119-128 Apr. 3, 1992.
Fanidi, A., E. Harrington, G. Evan, Cooperative Interaction Between C-CMYC and BCL-2 Proto-Oncogenes, Nature, vol. 359, Oct. 8, 1992.
Henderson, S., M. Rowe, C. Gregory, D. Carter, F. Wang, R. Longnecker, E. Kieff, A. Rickinson, Induction of BCL-2 Expression by Epstein-Barr Virus Latent Membrane Protein 1 Protects Infected B Cells From Programmed Cell Death, Cell, vol. 65, 1107-1115 Jun. 28, 1991.
Kurita, T., H. Namiki Serum Induced Cell Death, Zoological Science, vol. 10, 431-438 1993.
Kurita, T., H. Namiki, Apoptotic Cell Death Induced by Serum and its Prevention by Thiols, Journal of Cellular Physiology, vol. 161(1), 63-70 1994.
Kurita, T., H. Namiki, Comparison of Cytoxicity Among Sera From Various Sources, Cytologia, vol. 58, 445-452 1993.
Meister, A., S. Tate, Glutathione and Related Y-Glutamyl Compounds: Biosynthesis and Utlization, Annual Review of Biochemistry, vol. 45, 559-604 1976.
Rouach, E., D. Resnitsky, J. Lotem, L. Sachs, A. Kimchi, M. Oren, Wild-TYP P53 Induces Apoptosis of Myeloid Leukemic Cells That is Inhibited by Interleukin-6, Nature, vol. 352, Jul. 25, 1995.
Rubartelli, A., N. Bonifaci, R. Sitia, High Rates of Thioredoxin Secretion Correlate With Growth Arrest in Hepatoma Cells, Cancer Research, vol. 55, 675-680, Feb. 1, 1995.
Wyllie, A.H., J.F.R. Kerr, A.R. Currie, Cell Death: The Significance of Apoptosis, Internal Review of Cytology, vol. 68, 251-306 1980.
Ziegler, D.M., Role of Reversible Oxidation-Reduction of Enzyme Thiols-Disulfides in Metabolic Regulation, Annual Review of Biochemistry, vol. 54 305-329 1985.

Affiliated with

Also associated with

Rating

Technique for acceleration of apoptotic cell death does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Technique for acceleration of apoptotic cell death, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Technique for acceleration of apoptotic cell death will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1454663