tdcBC/pckA gene-inactivated microorganism and method of...

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

Reexamination Certificate

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Reexamination Certificate

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07485450

ABSTRACT:
The present invention provide a microorganism comprising an inactivated chromosomal tdcBC gene and an inactivated chromosomal pckA gene, which has remarkably improved productivity of L-threonine. Also, the present invention provides a method of producing L-threonine using the microorganism. The microorganism is prepared by incorporating by a recombination technique an antibiotic resistance gene into a pckA gene on the chromosome of a bacterial strain containing an L-threonine degradation-associated operon gene, tdcBC, which is inactivated. The microorganism has the effect of preventing degradation and intracellular influx of L-threonine due to the inactivation of the tdcBC operon gene, and includes more activated pathways for L-threonine biosynthesis. Therefore, the microorganism is useful for mass production of L-threonine because of being capable of producing L-threonine in high levels and high yields even in the presence of high concentrations of glucose.

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patent: 1347057 (2003-09-01), None
Ozaki et al. Agric. Biol. Chem. 47 No. 7 (1983) 1569-1576.
Benjamin Lewin Genes IV., 1990, p. 810 Definition of a “gene”.
Palmeros et al 2000 Gene 247 255-264.

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