Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Reexamination Certificate
2004-04-02
2009-02-03
Navarro, Mark (Department: 1645)
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
Reexamination Certificate
active
07485450
ABSTRACT:
The present invention provide a microorganism comprising an inactivated chromosomal tdcBC gene and an inactivated chromosomal pckA gene, which has remarkably improved productivity of L-threonine. Also, the present invention provides a method of producing L-threonine using the microorganism. The microorganism is prepared by incorporating by a recombination technique an antibiotic resistance gene into a pckA gene on the chromosome of a bacterial strain containing an L-threonine degradation-associated operon gene, tdcBC, which is inactivated. The microorganism has the effect of preventing degradation and intracellular influx of L-threonine due to the inactivation of the tdcBC operon gene, and includes more activated pathways for L-threonine biosynthesis. Therefore, the microorganism is useful for mass production of L-threonine because of being capable of producing L-threonine in high levels and high yields even in the presence of high concentrations of glucose.
REFERENCES:
patent: 4278765 (1981-07-01), Debabov et al.
patent: 5919694 (1999-07-01), Sugimoto et al.
patent: 6107063 (2000-08-01), Moeckel et al.
patent: 6420151 (2002-07-01), Eikmanns et al.
patent: 1347057 (2003-09-01), None
Ozaki et al. Agric. Biol. Chem. 47 No. 7 (1983) 1569-1576.
Benjamin Lewin Genes IV., 1990, p. 810 Definition of a “gene”.
Palmeros et al 2000 Gene 247 255-264.
Cho Jae Yong
Kim Dae Cheol
Lee Byoung Choon
Lee Jin Ho
Park Young Hoon
Archie Nina A
Arent & Fox LLP
CJ Cheiljedang Corporation
Navarro Mark
LandOfFree
tdcBC/pckA gene-inactivated microorganism and method of... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with tdcBC/pckA gene-inactivated microorganism and method of..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and tdcBC/pckA gene-inactivated microorganism and method of... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-4124210