TBG assay

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

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23915, 435 26, G01N 3114

Patent

active

041682076

ABSTRACT:
Method and compositions are provided for determining the thyroxine binding capacity of serum, the value finding use in determinations of free thyroxine index. A competitive assay is performed where antibody for T-3 (anti-triiodothyronine) and serum thyroxine binding sites compete for T-3(triiodothyronine) in the assay medium: the T-3 is present as free T-3 added in a predetermined amount and T-3 conjugated to an enzyme. By combining the materials in a buffered aqueous medium with enzyme substrates, and determining the rate of reaction by the changing concentration of a light absorptive substance over a predetermined period of time, the thyroxine binding capacity of the serum can be determined.

REFERENCES:
patent: 3817837 (1974-06-01), Rubenstein et al.
patent: 4040907 (1977-08-01), Ullman et al.
patent: 4043872 (1977-08-01), Blakemore et al.
Levy et al., J. Clin. Endocrinol. Metab., 35:565, 1972.

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