Targeted transfection of cells using a biotinylated dendrimer

Details Targeted transfection of cells using a biotinylated dendrimer Targeted transfection of cells using a biotinylated dendrimer

2007-03-20

2007-03-20

Martinell, James (Department: 1634)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

C435S455000, C536S023100

Reexamination Certificate

active

10240981

ABSTRACT:
The present invention relates to processes for the targeted transfection of cells, compositions which may be used for such processes, and corresponding pharmaceutical compositions for gene therapy. In particular, the invention relates to a process for introducing nucleic acid into cells comprising the steps of (a) mixing a nucleic acid with a dendrimer, in which a proportion of the dendrimer molecules are biotinylated; (b) mixing the resulting complex of nucleic acid and dendrimer with a second complex consisting of avidin or streptavidin and a biotinylated target-specific binding molecule; and (c) incubating the complex formed in step (b) with cells. Dendrimers which are well suited to the present invention include, for example, partially solvolysed polyamidoamine (PAMAM) dendrimers. Target-specific binding molecules are, in particular, cell-type-specific markers of the cell surface of the target cells.

REFERENCES:
patent: 4507466 (1985-03-01), Tomalia et al.
patent: 4558120 (1985-12-01), Tomalia et al.
patent: 4568737 (1986-02-01), Tomalia et al.
patent: 4587329 (1986-05-01), Tomalia et al.
patent: 4631337 (1986-12-01), Tomalia et al.
patent: 4694064 (1987-09-01), Tomalia et al.
patent: 4713975 (1987-12-01), Tomalia et al.
patent: 4737550 (1988-04-01), Tomalia et al.
patent: 4857599 (1989-08-01), Tomalia et al.
patent: 4871779 (1989-10-01), Killat et al.
patent: 5714166 (1998-02-01), Tomalia et al.
patent: 0 274 180 (1993-11-01), None
patent: 0 234 408 (1995-03-01), None
patent: 0 247 629 (1995-10-01), None
patent: WO 84/02705 (1984-07-01), None
patent: WO 93/19768 (1993-10-01), None
patent: WO 95/02397 (1995-01-01), None
Molecular Biology Reagents/Protocols 1992, United States Biochemical Corporation, 1991, Cleveland, Ohio, pp. 218-219.
Schoeman et al.,J. Drug Target, 2: 509-516 (1995).
Tang et al.,Biconjug. Chemistry, 7: 703-714 (1996).
Tomalia et al.,Angew Chem. Int. Ed., 29: 138 (1990).
Wilbur et al.,Bioconjug. Chem., 9: 813-825 (1998).

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