Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1995-02-27
1998-06-16
Robinson, Douglas W.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
4352351, 530300, 530324, 530326, 530350, 536 231, 536 245, C07K 708, C07K 1400, C12N 1511, C12N 1585
Patent
active
057668990
ABSTRACT:
Disclosed is a receptor-mediated complex that selectively delivers nucleic acid into hepatocytes. Circumsporozoite (CS) protein is the targeting ligand that recognizes a receptor expressed on the liver cell surface. The CS ligand is complexed with a polylysine component that can bind nucleic acid. The level of gene expression is greatly enhanced when the complex is cotransfected with adenovirus. Using the present invention, a reporter gene was successfully transferred into a number of different cell lines that express high levels of receptor. The ability to introduce nucleic acid into specific mammalian cells is an important therapy for numerous diseases such as cancer, malaria and hepatitis.
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Ding Zhi-Ming
Kuo M. Tien
Board of Regents , The University of Texas System
Nelson Amy J.
Robinson Douglas W.
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