Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1994-03-07
1997-04-29
Horlick, Kenneth R.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
514 44, 536 231, C12P 1934, C07H 2104, A61K 4800
Patent
active
056248245
ABSTRACT:
It has been discovered that any RNA can be targeted for cleavage by RNAase P from eukaryotic cells, for example, human RNAase P, using a suitably designed oligoribonucleotide ("external guide sequence", or EGS) to form a hybrid with the target RNA, thereby creating a substrate for cleavage by RNAase P in vitro. The EGS hydrogen bonds to the targeted RNA to form a partial tRNA like structure including the aminoacyl acceptor stem, the T stem and loop, and part of the D stem. The most efficient EGS with human RNAase P is the EGS in which the anticodon stem and loop was deleted. Modifications can also be made within the T-loop. Methods are also disclosed to randomly select and to express a suitable EGS in vivo to make a selected RNA a target for cleavage by the host cell RNAase P, thus preventing expression of the function of the target RNA. The methods and compositions should be useful to prevent the expression of disease-causing genes in vivo.
REFERENCES:
patent: 4868116 (1989-09-01), Morgan et al.
patent: 4980286 (1990-12-01), Morgan et al.
patent: 4987071 (1991-01-01), Cech et al.
patent: 5168053 (1992-12-01), Altman et al.
Altman, S., et al., "Catalysis by the RNA subunit of RNase P-a minireview," Gene 82:63-64 (1989).
Altman, Sidney, "Ribonuclease P: An Enzyme with a Catalytic RNA Subunit," Advances in Enzymology ed. Alton Meister, 62:1-36 (John Wiley & Sons 1989).
Baer, Madeline, et al., "Structure and Transcription of a human gene for H1 RNA component of human RNase P," Nucleic Acids Research 18:97-103 (1989).
Beaudry and Joyce, Science 257;635 (1992).
Berzal-Heranz, et al., Genes and Dev. 6:129 (1992).
Bartkiewicz, et al., Genes and Dev. 3:488-499 (1989).
Branch, Andrea D., et al., "An Ultraviolet-Sensitive RNA Structural Element in a Viroid-Like Domain of the Hepatitis Delta Virus," Science 243:649-652 (1989).
Chowrira, et al., Nature 354:320 (1991).
Das, et al., EMBO 7:503-512 (1988).
Doersen, et al., J. Biol. Chem. 260:5942 (1985).
Forster, Anthony C., and Sidney Altman, "External Guide Sequences for an RNA Enzyme," Science 49:783-786 (1990).
Forster and Symons, Cell 50:9-16 (1987).
Green, et al., Nature 347:406 (1990).
Guerrier-Takada, Cecilia, et al., "Specific Interactions in RNA Enzyme-Substrate Complexes," Science 246:1578-1584 (1989).
Guerrier-Takada, Cecilia and Sidney Altman, "Catalytic Activity of an RNA Molecule Prepared by Transcription in vitro," Science 223:285-286 (1984).
Guerrier-Takada, Cecilia et al., "The RNA Moiety of Ribonuclease P is the Catalytic Subunit of the Enzyme," Cell 35:849-857 (1983).
Lawrence, Nathan P. and Sidney Altman, "Site-directed Mutagenesis of M1 RNA, the RNA Subunit of Escherichia coli Ribonuclease P," J. Mol. Biol. 191:163-175 (1986).
Lee, Jae-Yong and David R. Engelke, "Partial Characterization of an RNA Component That Copurifies with Saccharomyces cerevisiae RNase P," Molecular and Cellular Biology 9(6): 2536-2543 (1989).
Li, Ying, et al., "Targeted cleavage of mRNA in vitro by RNase P from Escherichia coli Proc. Natl. Acad." 89:3185-3189 (1992).
McClain, William H., et al., "Model Substrates for an RNA Enzyme," Science 238:527-530 (1987).
Mills, Donald R., et al., "Q.beta. Replicase: Mapping the Functional Domains of an RNA-dependent RNA Polymerase," J. Molecular Biology 205:751-764 (1988).
Pace, Norman R., et al., "Phylogenetic Comparative analysis and the secondary structure of ribonuclease P RNA--a review," Gene 82:65-75 (1989).
Pace, Norman R., et al., "Ribonuclease P: Function and Variation," J. of Biol. Chem. 265(7):3587-3590 (1990).
Perreault and Altman, J. Mol. Biol. 226:399-409 (1992).
Pyle, et al., Proc. Natl. Acad. Sci. USA 87:8187-8191 (1990).
Roizman, Cell 16:481-494 (1979).
Rossi, J., et al., J. Cell Biol. (Supp. 14A, D428) (1990) (Abs tract).
Sarver, Nava, et al., "Ribozymes as Potential Anti-HIV-1 Therapeutic Agents," Science 247:1222-1225 (1990).
Sharmeen, et al., J. Virol. 62:2674-2679 (1988).
Surratt, Christopher K., et al., "Processing of a Synthetic tRNA Precursor Model by E. coli RNase P and M1 RNA," Molecular Biology of RNA 79-88 (Alan R. Liss, Inc. 1989).
Tuerk and Gold, Science 249:505 (1990).
Wagner, et al., Proc. Natl. Acad. Sci. 78:1441-1445 (1981).
Wigler, et al., Proc. Natl. Acad. Sci. USA 76:1373-1376, (1979).
Wu, Huey-Nan and Michael M. C. Lai, "Reversible Cleavage and Ligation of Hepatitis Delta Virus RNA," Science 243:652-655 (1989).
Yuan, Yan, et al., "Targeted cleavage of mRNA by human RNase P," Proc. Natl. Acad. Sci. 89:8006-8010 (1982).
Yuan et al., EMBO J. 14(1), 159-168 (1995).
Yuan et al, Science 263, 1269-1273 (Mar. 1994).
Altman, J. Biol. Chem. 265:20053-20056 (1990).
Altman Sidney
Guerrier-Takada Cecilia
Liu Fenyong
Yuan Yan
Horlick Kenneth R.
Pabst Patrea L.
Yale University
LandOfFree
Targeted cleavage of RNA using eukaryotic ribonuclease P and ext does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Targeted cleavage of RNA using eukaryotic ribonuclease P and ext, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Targeted cleavage of RNA using eukaryotic ribonuclease P and ext will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-705006