Targeted chromosomal genomic alterations with modified...

Chemistry: molecular biology and microbiology – Process of mutation – cell fusion – or genetic modification – Introduction of a polynucleotide molecule into or...

Reexamination Certificate

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C435S440000, C435S463000, C435S471000, C435S006120, C536S023100, C536S024200, C536S024300

Reexamination Certificate

active

06936467

ABSTRACT:
Presented are methods and compositions for targeted chromosomal genomic alterations using modified single-stranded oligonucleotides. The oligonucleotides of the invention have at least one modified nuclease-resistant terminal region comprising phosphorothioate linkages, LNA analogs or 2′-O-Me base analogs.

REFERENCES:
patent: 5565350 (1996-10-01), Kmiec
patent: 5731181 (1998-03-01), Kmiec
patent: 5801154 (1998-09-01), Baracchini et al.
patent: 5912340 (1999-06-01), Kutyavin
patent: 6004804 (1999-12-01), Kumar
patent: 6136601 (2000-10-01), Meyer, Jr. et al.
patent: 6271360 (2001-08-01), Metz
patent: WO 99/14226 (1999-03-01), None
patent: WO 00/56748 (2000-09-01), None
patent: WO 00/66604 (2000-11-01), None
Ronald G. Crystal, SCIENCE, vol. 270, Oct. 20, 1995, pp. 404-410.
Inder M. Verma et al., NATURE, vol. 389, Sep. 18, 1997, pp. 239-242.
Howard B Gamper et al., Nucleic Acids Research, 2000, vol. 28, No. 21, pp. 4332-4339.
Kenneth W. Culver et al., Nature Biotechnology, vol. 17,Oct. 1999, pp. 989-993.
RJ Yanez et al., Gene Therapy, (1998), 5, pp. 149-159.
Eugene H. Kaji, et al., JAMA, Feb. 7, 2001, vol. 285, No. 5, pp. 545-550.
Jon R. Sayers et al., Nucleic Acids Research, vol. 16, No. 3, 1988, pp. 791-801.
Alexeev & Yoon, “Stable and inheritable changes in genotype and phenotype of albino melanocytes induced by an RNA-DNA oligonucleotide,”Nature Biotech.16:1343-1346 (1998).
Campbell et al., “Homologous recombination involving small single-stranded oligonucleotides in human cells,”New Biologist1:223-227 (1989).
Chan & Glazer, “Triplex DNA: fundamentals, advances, and potential applications for gene therapy,”J. Mol. Med.75:267-282 (1997).
Chan et al., “Targeted correction of an episomal gene in mammalian cells by a short DNA fragment tethered to a triplex-forming oligonucleotide,”J. Biol. Chem.274:11541-11548 (1999).
Cole-Strauss et al., “Correction of the mutation responsible for sickle cell anemia by an RNA-DNA oligonucleotide,”Science273:1386-1389 (1996).
Culver et al., “Correction of chromosomal point mutations in human cells with bifunctional oligonucleotides,”Nat. Biotechnol.17:989-993 (1999).
Gamper et al., “The DNA strand of chimeric RNA/DNA oligonucleotides can direct gene repair/conversion activity in mammalian and plant cell-free extracts,”Nucleic Acids Res.28:4332-4339 (2000).
Igoucheva et al., “Targeted gene correction by small single-stranded oligonucleotides in mammalian cells,”Gene Therapy8:391-399 (2001).
Kren et al., “Correction of the UDP-glucuronosyltransferase gene defect in the Gunn rat model of Crigler-Najjar syndrome type I with a chimeric oligonucleotide,”Proc. Natl. Acad. Sci. USA96:10349-10354 (1999).
Kunzelmann et al., “Gene targeting of CFTR DNA in CF epithelial cells,”Gene Ther.3:859-867 (1996).
Moerschell et al., “Transformation of yeast with synthetic oligonucleotides,”Proc. Natl. Acad. Sci. USA85:524-528 (1988).
Rando et al., “Rescue of dystrophin expression inmdxmouse muscle by RNA/DNA oligonucleotides,”Proc. Natl. Acad. Sci. USA97:5363-5368 (2000).
Vasquez et al., “Chromosomal mutations induced by triplex-forming oligonucleotides in mammalian cells,”Nucl. Acids Res.27:1176-1181 (1999).
Vasquez et al., “Specific mutations induced by triplex-forming oligonucleotides in mice,”Science290:530-532 (2000).
Yamamoto et al., “Strand-specificity in the transformation of yeast with synthetic oligonucleotides,”Genetics131:811-819 (1992).
Yanez & Porter, “Therapeutic gene targeting,”Gene Therapy5:149-159 (1998).
Xu et al., “Activation of human γ-globin gene expression via triplex-forming oligonucleotide (TFO)- directed mutations in the γ-globin gene 5′ flanking region,”Gene242:219-228 (2000).

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