Drug – bio-affecting and body treating compositions – Whole live micro-organism – cell – or virus containing – Genetically modified micro-organism – cell – or virus
Reexamination Certificate
2005-06-28
2005-06-28
Nguyen, Dave T. (Department: 1632)
Drug, bio-affecting and body treating compositions
Whole live micro-organism, cell, or virus containing
Genetically modified micro-organism, cell, or virus
C435S091400, C435S320100
Reexamination Certificate
active
06911199
ABSTRACT:
Modification of internal sites of the adenovirus fiber protein and hexon protein permit effective targeting of adenovirus vectors. Accessible sites to redirect adenovirus targeting were identified. The HVR5 loop of the hexon protein and the HI loop of the fiber protein (knob) were highly permissive for the insertion of foreign protein sequences, which apparently did not impact on the viability and productivity of corresponding viruses. Accessibility and functionality of the epitope strongly depend on the size of the neighboring spacers. Other results suggest that short targeting peptides can be effectively fused to the C-terminus of the fiber protein. In a specific embodiment, a series of adenovirus vectors modified at the HVR5 site, the fiber protein HI loop, or the fiber protein C-terminus to target urokinase-type plasminogen activator receptor bearing cells were prepared. Such vectors are particularly useful for targeting the vasculature, e.g., for gene therapy of cancers or cardiovascular conditions.
REFERENCES:
patent: 5846782 (1998-12-01), Wickham et al.
patent: 5980886 (1999-11-01), Kay
patent: 6120765 (2000-09-01), Hibino
patent: 6127525 (2000-10-01), Crystal
patent: 6287857 (2001-09-01), O'Riordan et al.
patent: WO 95/05201 (1995-02-01), None
patent: WO 95/26412 (1995-10-01), None
patent: WO 96/26281 (1996-08-01), None
patent: WO 97/20051 (1997-06-01), None
patent: WO 98/07865 (1998-02-01), None
patent: WO 98/07877 (1998-02-01), None
patent: WO 98/40509 (1998-09-01), None
Crompton J, Expression of a Foreign Epitope on the Surface of the Adenovirus Hexon, Journal of General Virology, GB, Society for General Microbiology, Reading, vol. 75, pp. 133-139.
Krasnykh Victor et al., Characterization of an Adenovirus Vector Containing a Heterologous Peptide Epitope in the HI Loop of the Fiber Knob, Journal of Virology, Mar. 1998, pp. 1844-1852.
Vigne Emmanuelle et al., RGD Inclusion in the Hexon Monomer Provides Adenovirus Type 5-Based Vectors with a Fiber Knob-Independent Pathway for Infection, Journal of Virology, Jun. 1999, vol. 73, No. 6, pp. 5156-5161.
Wickenham T J, Adenovirus Targeted to Heparan-containing Receptors Increases its Gene Delivery Efficiency to Multiple Cell Types, Biotechnology. The International Monthly for Industrial Biology, US, Nature Publishing Co., NY, vol. 14, No. 11, pp. 1570-1573.
Dedieu Jean-Francois
Latta Martine
Perricaudet Michel
Vigne Emmanuelle
Yeh Patrice
Aventis Pharma S.A.
Nguyen Dave T.
Synnestvedt & Lechner LLP
LandOfFree
Targeted adenovirus vectors for delivery of heterologous genes does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Targeted adenovirus vectors for delivery of heterologous genes, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Targeted adenovirus vectors for delivery of heterologous genes will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3487683