Target-sensitive immunoliposomes- preparation and characterizati

Details Target-sensitive immunoliposomes- preparation and characterizati Target-sensitive immunoliposomes- preparation and characterizati

1987-02-09

1990-09-18

Warden, Robert J.

Drug, bio-affecting and body treating compositions

Lymphokine

424 8591, 424 86, 424427, 424450, 436829, 530389, 530390, A61K 950, A61K 3942, A61K 3944

Patent

active

049577352

ABSTRACT:
Novel target-sensitive immunoliposomes were prepared and characterized. In this invention, target specific binding of antibody-coated liposomes was sufficient to induce bilayer destabilization, resulting in a site-specific release of liposome contents.
Unilamellar liposomes were prepared by using a small quantity of palmitoyl IgG (pIgG) to stabilize the bilayer phase of the unsaturated phosphatidylethanol amine (PE) which by itself does not form stable liposomes. A mouse monoclonal IgG antibody to the glycoprotein D (gD) of Herpes Simplex Virus (HSV) and dioleoyl PE were used in one preferred embodiment.
In another preferred embodiment, potentially cytotoxic antiviral drugs were entrapped in target sensitive (TS) immunoliposomes and delivered to HSV infected cells. Potency was as much as 1000 times superior to the free drug and cytotoxicity was decreased by as much as 3000 fold.

REFERENCES:
patent: 3887698 (1975-11-01), McConnell
patent: 4193983 (1980-03-01), Ullman et al.
patent: 4235792 (1980-11-01), Hsia et al.
patent: 4342739 (1982-08-01), Kakimi et al.
patent: 4342826 (1982-08-01), Cole
patent: 4372745 (1983-02-01), Mandle et al.
patent: 4480041 (1984-10-01), Myles et al.
patent: 4483921 (1984-11-01), Cole
patent: 4517303 (1985-05-01), Freytag et al.
patent: 4668638 (1987-05-01), Janoff et al.
patent: 4671958 (1987-06-01), Rodwell et al.
patent: 4708933 (1987-11-01), Huang et al.
patent: 4731324 (1988-03-01), Huang et al.
patent: 4789633 (1988-12-01), Huang et al.
Ho et al., Journ. Immunol., 134, 4035-4040, 1985.
Ho et al, Biochemistry, 25, 5500-5506, 1986.
Huang et al, Journ. Biol. Chem., 255, 8015-8018, 1980.
Petrossian et al., Biochimica et Biophysica Acta. (1984): 217-227.
Alving et al., Biochemistry 8 (1969): 1582.
Kinsky et al., Biochemistry 8 (1969): 4149.
Alving et al., Liposomes (Marcel Dekker 1983): 209-287.
Szoka et al., Annual Rev. Biophys. & Biogen. 9 (1980): 467.
Deamer et al., Liposomes (Marcell Dekker 1983): 27-51.
Reiss-Husson, Journal of Molecular Biology 25 (1967): 363.
Rand et al., Chem. Phys. Lipids 6 (1971): 333.
Enoch et al., PNAS 76 (1979): 145.
Eisen et al., Meth. Immunology Immunochemistry 1 (1967): 351.
Warr, Antibody As A Tool . . . (Wiley, 1982): 59-96.

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