Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Patent
1997-10-14
2000-05-16
Jones, W. Gary
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
435 6, 536 231, 536 243, 935 76, 935 77, 935 78, C12P 1934, C12Q 168, C07H 2102, C12N 1500
Patent
active
060636041
ABSTRACT:
A method is provided for the rapid, substantially isostatic, segregation and amplification of the sequence information of a target nucleic acid sequence positioned within a single- or double-stranded polynucleotide. The method is based on the serial generation of double-stranded DNA engineered to contain terminal nicking sites, nicking of those sites, and extensions from those nicks, thereby displacing any existing polynucleotides. Further provided is a method for detecting polynucleotides using the method of the invention. A kit combining the components commonly used in practicing the method of the invention is also provided.
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Blassak Michele L.
Mueller Reinhold
Wick James F.
Wilkosz Richard K.
Jones W. Gary
Molecular Biology Resources, Inc.
Whisenant Ethan
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