Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving virus or bacteriophage
Patent
1995-05-16
1997-05-20
Marschel, Ardin H.
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving virus or bacteriophage
435 6, 435 911, 435 912, 435 9121, 435 913, 435 915, 435 9151, 435810, 436501, 536 221, 536 231, 536 241, 536 243, 536 2431, 536 2432, 536 2433, 536 253, 536 254, 935 77, 935 78, C12Q 168, C07H 2102, C07H 2104
Patent
active
056311290
ABSTRACT:
This invention relates to the use of functional reporter molecules in the detection and measurement of RNA sequences in a sample, as a determination, for example, of pathogenic disease existence or potential. The invention is predicated on the utilization of nucleotide sequences, one having a probe sequence linked to a sequence capable of initiating replication by an RNA-dependent RNA polymerase. The other is capable of hybridizing to a strand separated from the extension product of the first nucleotide sequence after hybridization to a specific target sequence. The extension product of the second hybridized nucleotide sequence serves as a template source for autocatalytic replication by the RNA-dependent RNA polymerase. The replication products are detected as a means for detection of nucleic acid sequences.
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Feix, et al., "Primer Directed Initiation of RNA Synthesis Catalysed by Q.beta. Replicase", Biochem. Biophys. Res. Comm. 65, 503-509 (1975).
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Miyake, et al., "Grouping of RNA Phages Based on the Template Specificity of Their RNA Replicases", Proc. Natl. Acad. Sci. USA, 68, 2022-2024 (1971).
Murakawa, et al. "Direct Detection of HIV-1 RNA from AIDS and ARC Patient Samples", DNA 7, 287-295 (1988).
Chu Barbara C.
Joyce Gerald F.
Orgel Leslie E.
Marschel Ardin H.
Ramos Robert T.
Reiter Stephen E.
The Salk Institute for Biological Studies
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