Target-dependent synthesis of an artificial gene for the synthes

Details Target-dependent synthesis of an artificial gene for the synthes Target-dependent synthesis of an artificial gene for the synthes

1989-05-26

1992-05-12

Wax, Robert A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 91, 4351723, 435805, 436501, 436815, 536 27, 935 2, 935 9, 935 17, 935 18, 935 19, 935 78, 935 88, C12Q 168, C07H 2104

Patent

active

051127342

ABSTRACT:
This invention pertains to an improved method for detecting a nucleic acid target sequence with a replicatable RNA reporter system. Two polymerase-mediated reactions are used to generate a target-specific gene containing a DNA sequence for a replicatable RNA. Transcription of the target-specific gene yields a replicatable RNA which is amplified by replication. Synthesis of the gene and the replicatable RNA is strictly dependent upon specific interaction with the target sequence. Consequently, the amplified signal (RNA) is target-dependent and background signal is reduced.

REFERENCES:
patent: 4683195 (1987-07-01), Mullis et al.
patent: 4683202 (1987-07-01), Mullis
patent: 4786600 (1988-11-01), Kramer et al.
patent: 4800159 (1989-01-01), Mullis et al.
Miele (1982) Ph.D. Thesis, University Microfilms, Ann Arbor, Michigan.
Kwoh et al., Proc. Natl. Acad. Sci. USA 86:1173-1177 (Feb., 1989).
Miele et al., J. Mol. Biol., 171:281-295 (1983).
Lizardi et al., Bio/Technology 6:1197-1202 (Oct. 1988).

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