Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide alters fat – fatty oil – ester-type wax – or...
Patent
1995-04-05
1998-03-03
McElwain, Elizabeth
Multicellular living organisms and unmodified parts thereof and
Method of introducing a polynucleotide molecule into or...
The polynucleotide alters fat, fatty oil, ester-type wax, or...
536 236, 536 241, 435 691, 4351723, 4352404, 4352523, 4353201, A01H 500, C12N 1512, C12N 1582
Patent
active
057237549
ABSTRACT:
Tapetum-specific promoters, designated A3 and A9 and capable of driving expression of 12.9 kDa and 11.6 kDa proteins in Arabidopsis thaliana and related proteins within the family Brassicaceae, have been discovered, isolated and cloned. The promoters can be used to drive male sterility DNA such as that coding for a nuclease, protease or glucanase. Alternatively or in addition, male sterility can be achieved by disrupting the proper expression of the A3 and/or A9 genes, for example by transcribing RNA which is antisense to the RNA normally transcribed from the A3 and A9 genes, or by expressing DNA coding for a ribozyme specific for at least one of the A3 and A9 genes.
REFERENCES:
Scott, R., et al.; "Identification of Genes Exhibiting Brassica napus;" Journal of Experimental Botany; vol. 41, 1990, Suppl., P5-3.
Hodge, R. P., et al.; "A9 a Tapetum-Specific Gene;" Journal Experimental Botany; vol. 42, 1991, 238 Suppl.; Meeting Apr. 7-12; 1991; p. 46.
Paul, W. et al.; "Aspects of the Molecular Biology of Anther Development"; Journal Experimental Botany; vol. 42, 1991, 238 Suppl.; Meeting Apr. 7-12; 1991; p. 40.
Roberts, M., et al.; "Isolation and Characterization of Pollen Specific Promoters from Arabidopsis thaliana"; Journal Experimental Botany; vol. 41, 1990, Suppl., P5-2.
Mariani, C., et al.; "Induction of Male Sterility in Plants by a Chimaeric Ribonuclease Gene;" Nature; vol. 347; 25 Oct. 1990; pp. 737-741.
Barghchi, M. et al.; "Genetic Engineering of Arabidopsis;" Abstracts VIIth International Congress on Plant Tissue and Cell Culture; 1990, Jun. 24-29, Amsterdam, NL; p. 46.
Kim et al. (1994) Plant Mol Biol 24:105-117.
Draper John
Paul Wyatt
Scott Roderick John
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