Tapetum-specific promoters

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide contains a tissue – organ – or cell...

Reexamination Certificate

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C536S024100, C800S298000, C435S252300, C435S320100, C435S419000

Reexamination Certificate

active

07078587

ABSTRACT:
The present invention relates to nucleic acid sequences encoding tapetum specific promoters, for use in Artificial Male Sterility systems in plants. In particular, the promoters may be the pMAC2 promoter; the pMAC20 promoter; or promoter sequences which naturally controls the expression of a coding sequence substantially homologous to the MAC2 or MAC20 coding sequences. Also provided are regulatory elements of the promoters; plant cells and plants transformed with the promoter sequences.

REFERENCES:
patent: 5635384 (1997-06-01), Walsh et al.
patent: 5837850 (1998-11-01), Huffman
patent: WO 97/38116 (1997-10-01), None
Kim et al. A 20 nucleotide upstream element is essential for the nopaline synthase (nos) promoter activity. Plant Molecular Biology, 1994, vol. 24, pp. 105-117.
Eyal et al. Pollen specificity elements reside in 30 bp of the proximal promoters of two pollen-expressed genes. The Plant Cell, Mar. 1995, vol. 7, pp. 373-384.
Database GenEMBL Accession No. AW057058, Walbot, V., “660008812.yl 660—Mixed stages of anther and pollen Zea Mays cDNA, mRNA sequence,” (Oct. 6, 1999).
Database GenEMBL Accession No. AW562916, Walbot, V., “660069F07.x1 660—Mixed stages of anther and pollen Zea mays cDNA, mRNA sequence” (Mar. 15, 2000).
International Search Report for International Application No. PCT/GB00/01789, mailed Apr. 3, 2001.

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