Tagged ligands for enrichment of rare analytes from a mixed...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving antigen-antibody binding – specific binding protein...

Reexamination Certificate

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C435S002000, C435S007200, C435S007500, C435S286500, C435S287200, C435S971000, C435S973000, C436S501000, C436S518000, C436S056000, C436S064000, C436S175000, C436S177000, C436S010000, C436S524000, C436S528000, C436S532000, C422S417000, C422S502000, C422S504000

Reexamination Certificate

active

08008032

ABSTRACT:
Method of enriching specific cells from cellular samples are disclosed, comprising contacting in solution a cellular sample with affinity-tagged ligands (ATLs) each comprising a first ligand linked to an affinity tag, wherein the ligand selectively binds a cellular marker of the rare cells and the affinity tag can be selectively captured by a capture moiety, wherein the affinity tags do not comprise a magnetic particle; and flowing the sample through a microfluidic device comprising the capture moiety to selectively retain ATL-bound cells. Methods for enriching circulating tumor cells, and devices for enriching specific cells from cellular samples are also disclosed.

REFERENCES:
patent: 5635363 (1997-06-01), Altman et al.
patent: 5693539 (1997-12-01), Miltenyi et al.
patent: 5707799 (1998-01-01), Hansmann et al.
patent: 5715946 (1998-02-01), Reichenbach
patent: 5837115 (1998-11-01), Austin et al.
patent: 6156270 (2000-12-01), Buechler et al.
patent: 6190870 (2001-02-01), Schmitz et al.
patent: 6471860 (2002-10-01), Miltenyi et al.
patent: 6613525 (2003-09-01), Nelson et al.
patent: 6645731 (2003-11-01), Terstappen et al.
patent: 6913697 (2005-07-01), Lopez et al.
patent: 7030228 (2006-04-01), Schmitz et al.
patent: 7150812 (2006-12-01), Huang et al.
patent: 7166423 (2007-01-01), Miltenyi et al.
patent: 7190818 (2007-03-01), Ellis et al.
patent: 7262269 (2007-08-01), Lam et al.
patent: 7428325 (2008-09-01), Douglass et al.
patent: 7783098 (2010-08-01), Douglass et al.
patent: 7785810 (2010-08-01), Chen
patent: 2003/0159999 (2003-08-01), Oakey et al.
patent: 2004/0005582 (2004-01-01), Shipwash
patent: 2004/0018611 (2004-01-01), Ward et al.
patent: 2004/0048360 (2004-03-01), Wada et al.
patent: 2004/0144651 (2004-07-01), Huang et al.
patent: 2005/0266433 (2005-12-01), Kapur et al.
patent: 2005/0282220 (2005-12-01), Prober et al.
patent: 2006/0024756 (2006-02-01), Tibbe et al.
patent: 2006/0134599 (2006-06-01), Toner et al.
patent: 2006/0151265 (2006-07-01), Honjou et al.
patent: 2006/0226433 (2006-10-01), Kawano
patent: 2006/0252054 (2006-11-01), Lin et al.
patent: 2006/0252087 (2006-11-01), Tang et al.
patent: 2006/0266692 (2006-11-01), Foster et al.
patent: 2006/0285996 (2006-12-01), Ohman et al.
patent: 2007/0020772 (2007-01-01), Cao et al.
patent: 2007/0026381 (2007-02-01), Huang et al.
patent: 2007/0026414 (2007-02-01), Fuchs et al.
patent: 2007/0059680 (2007-03-01), Kapur et al.
patent: 2007/0160503 (2007-07-01), Sethu et al.
patent: 2007/0212737 (2007-09-01), Clarke et al.
patent: 2009/0136982 (2009-05-01), Tang et al.
patent: 2010/0059414 (2010-03-01), Sturm et al.
patent: 2010/0167337 (2010-07-01), Tsinberg et al.
patent: 2010/0248358 (2010-09-01), Yoshioka
patent: 1597353 (2010-11-01), None
patent: WO 02/12896 (2002-02-01), None
patent: WO 02/12896 (2002-02-01), None
patent: WO 2006/108087 (2006-10-01), None
patent: WO 2007/126938 (2007-11-01), None
patent: WO 2008/017871 (2008-02-01), None
Nagrath et al. (Isolation of rare circulating tumour cells in cancer patients by microchip technology (Nature 450: (20/27): 1235-1238 (Dec. 2007)).
Aggarwal et al. (A combinatorial approach to the selective capture of circulating malignant epithelial cells by peptide ligands, Biomaterials 26: 6077-6086 (2005)).
Aggarwal, et al. A combinatorial approach to the selective capture of circulating malignant epithelial cells by peptide ligands. Biomaterials. Oct. 2005;26(30):6077-86.
Fan, et al. Biotin derivatives of methotrexate and folate. Synthesis and utilization for affinity purification of two membrane-associated folate transporters from L1210 cells. J Biol Chem. Aug. 15, 1991;266(23):14862-5.
He, et al. In vivo quantitation of rare circulating tumor cells by multiphoton intravital flow cytometry. Proc Natl Acad Sci U S A. Jul. 10, 2007;104(28):11760-5.
Kurihar, et al. Imaging brain tumors by targeting peptide radiopharmaceuticals through the blood-brain barrier. Cancer Res. Dec. 15, 1999;59(24):6159-63.
Li, et al. Identification and characterization of a novel peptide ligand of epidermal growth factor receptor for targeted delivery of therapeutics. FASEB J. Dec. 2005;19(14):1978-85.
Nagrath, et al. Isolation of rare circulating tumour cells in cancer patients by microchip technology. Nature. 2007; 450: 1235-1241 (with Supplemental pp. 1-10).
Sandoval, et al. Uptake and trafficking of fluorescent conjugates of folic acid in intact kidney determined using intravital two-photon microscopy. Am J Physiol Cell Physiol. Aug. 2004;287(2):C517-26.

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