Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Reexamination Certificate
1999-03-03
2001-09-18
Caputa, Anthony C. (Department: 1642)
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
C536S023500, C536S024500, C530S350000, C530S395000, C436S064000
Reexamination Certificate
active
06291663
ABSTRACT:
BACKGROUND OF THE INVENTION
1. Field of the Invention
The present invention relates generally to the fields of cellular biology and the diagnosis of neoplastic disease. More specifically, the present invention relates to a transmembrane serine protease termed Tumor Antigen Derived Gene-12 (TADG-12), which is overexpressed in ovarian carcinoma.
2. Description of the Related Art
Proteases have been associated directly with tumor growth, shedding of tumor cells and invasion of target organs. Individual classes of proteases are involved in, but not limited to (1) the digestion of stroma surrounding the initial tumor area, (2) the digestion of the cellular adhesion molecules to allow dissociation of tumor cells; and (3) the invasion of the basement membrane for metastatic growth and the activation of both tumor growth factors and angiogenic factors.
The prior art is deficient in the lack of the complete identification of the proteases overexpressed in carcinoma. Specifically, TADG-12, a novel transmembrane serine protease, has not been previously identified in either nucleic acid or protein form. The present invention fulfills this longstanding need and desire in the art.
SUMMARY OF THE INVENTION
The present invention discloses TADG-12, a new member of the Tumor Antigen Derived Gene (TADG) family. TADG-12 is a novel transmembrane serine protease overexpressed in ovarian carcinoma.
In one embodiment of the present invention, there is provided a DNA encoding a TADG-12 protein selected from the group consisting of: (a) isolated DNA which encodes a TADG-12 protein; (b) isolated DNA which hybridizes to isolated DNA of (a) above and which encodes a TADG-12 protein; and (c) isolated DNA differing from the isolated DNAs of (a) and (b) above in codon sequence due to the degeneracy of the genetic code, and which encodes a TADG-12 protein.
In another embodiment of the present invention, there is provided a vector capable of expressing the DNA of the present invention adapted for expression in a recombinant cell and regulatory elements necessary for expression of the DNA in the cell.
In yet another embodiment of the present invention, there is provided a host cell transfected with the vector of the present invention, the vector expressing a TADG-12 protein.
In still yet another embodiment of the present invention, there is provided a method of detecting expression of a TADG-12 mRNA, comprising the steps of: (a) contacting mRNA obtained from the cell with the labeled hybridization probe; and (b) detecting hybridization of the probe with the mRNA.
The TADG-12 gene product has several potential application 1) as a target; 2) for antisense inhibition of its expression; 3) for gene therapy; 4) for vaccination; and 5) for inhibition by small molecular protease protein inhibitors.
Other and further aspects, features, and advantages of the present invention will be apparent from the following description of the presently preferred embodiments of the invention given for the purpose of disclosure.
REFERENCES:
Tanimoto, et al, “Cloning and expression of TADG-15, a novel serine protease expressed in ovarian cancer” Proceeding of the American Association for Cancer Research, vol. 39, p. 648, 1998.*
O'Brien et al, “Cloning and expression of TADG-15, a novel serine protease expressed in ovarian cancer.”, Tumor Biology, Suppl 2, p. 33, 1998.
O'Brien Timothy J.
Underwood Lowell J.
Adler Benjamin Aaron
Board of Trustees of the University of Arkansas
Canella Karen A.
Caputa Anthony C.
LandOfFree
TADG-12: a novel transmembrane serine protease overexpressed... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with TADG-12: a novel transmembrane serine protease overexpressed..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and TADG-12: a novel transmembrane serine protease overexpressed... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2532748