Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease
Patent
1990-03-01
1990-08-07
Mays, Thomas D.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Transferase other than ribonuclease
4351723, 435235, 43525233, 435320, 935 14, 935 29, 935 73, C12N 912, C12N 1500, C12N 120, C12N 700
Patent
active
049467867
ABSTRACT:
1Method for production of a composition consisting essentially of a T7-type DNA polymerase and thioredoxin. The method includes culturing a cell containing plasmid DNA encoding a T7-type DNA polymerase to express the T7-type DNA polymerase from the plasmid DNA, and purifying the T7-type DNA polymerase expressed from the cell to reduce the exonuclease activity associated with the T7-type DNA polymerase compared to the level of exonuclease activity associated with a corresponding naturally-occurring T7-type DNA polymerase.
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Richardson Charles C.
Tabor Stanley
Mays Thomas D.
President and Fellows of Harvard College
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