T.sub.3 Uptake polarization fluoroimmunoassay

Chemistry: physical processes – Physical processes – Crystallization

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23923, 424 15, 435 7, 435 8, G01N 3352, G01N 3354, G01N 3378

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043470599

ABSTRACT:
A T.sub.3 uptake polarization fluoroimmunoassay where a sample is assayed by contacting a serum sample with T.sub.3 to form a solution, contacting the solution with a separating agent and incubating the same, separating T.sub.3 bound to the separating agent from the serum sample, contacting the T.sub.3 bound to the separating agent with an antibody against T.sub.3, incubating and separating the agent-anti-body combination, separating free anti-body and contacting the free antibody with fluorescent labeled T.sub.3 and fluorometrically measuring the fluorescence polarization.

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