Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Using tissue cell culture to make a protein or polypeptide
Patent
1984-02-06
1987-12-15
Wiseman, Thomas G.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Using tissue cell culture to make a protein or polypeptide
435 91, 4351723, 536 27, 935 12, 935 15, C12P 2102, C12P 1934, C12N 1500, C07H 1512
Patent
active
047133325
ABSTRACT:
The invention provides a nucleic acid having a sequence which encodes a polypeptide that is at least part of a T cell antigen receptor. This encoded sequence is about 936 nucleotides in length and preferably is a human T cell antigen receptor. The nucleic acid sequence of one embodiment of the invention is shown in FIG. 3.
The nucleic acid sequence may be used as a probe to determine whether an unknown cell, e.g., a tumor cell, is a T cell.
Polypeptides encoded by the nucleic acid sequence include about 312 amino acids and are at least part of a T cell antigen receptor. They include at least one sequence which over 21 contiguous amino acids has greater than about 35% homology with mouse and human immunoglobin .lambda. light chains.
Antibody to the polypeptide may be prepared and used to identify T cell antigen receptor and to determine whether an unknown cell, e.g., a tumor cell, is a T cell.
REFERENCES:
Berzofsky, 1983, "Promising Clues to Reception Genes and Molecules", Immunology Today, vol. 4, pp. 299-301.
Hood et al., 1978, Immunology, Benjamin/Cummings Publishing Co., Menlo Park, pp. 7-8.
Kappler et al., 1983, "The Mouse T Cell Receptor: Comparison of MHC-Restricted Receptors on Two T Cell Hybridomas", Cell, vol. 34, 727-737.
Acuto et al., 1983, "The Human T Cell Receptor: Appearance in Ontogeny and Biochemical Relationship of .alpha.-and.beta.-Subunits . . .", Cell, vol. 34, 717-726.
Marx, 1983, "Likely T Cell Receptor Gene Cloned", Science, vol. 221, (Sep. 23), pp. 1278-1279.
Malissen et al., 1984, "Mouse T Cell Antigen Receptor: Structure and Organization of Constant and Joining Gene Segments Encoding B-Polypeptide", Cell, vol. 37, 1101-1110.
Kronenberg et al., 1983, "Finding the T-Cell Antigen Receptor: Past Attempts and Future Promise", Cell, vol. 34, 327-329.
McIntyre et al., 1983, "The Mouse T Cell Receptor: Structural Heterogeneity of Molecules of Normal T Cells Defined by Xenoantiserum", Cell, vol. 34, 739-746.
Mays Thomas D.
The Ontario Cancer Institute
Wiseman Thomas G.
LandOfFree
T cell specific CDNA clone does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with T cell specific CDNA clone, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and T cell specific CDNA clone will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1220016