Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of...
Patent
1996-12-31
1998-06-16
Scheiner, Laurie
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
4353391, 435347, 435355, 435372, 4353723, 435373, 435384, 435386, C12N 508
Patent
active
057669449
ABSTRACT:
A procedure for carrying out T cell differentiation of CD34+ stem cells in an in vitro culture of thymic epithelial fragments whereby the differentiated T cells achieve full immunocompetence. The invention also includes the procedure for differentiation of stem cells from HIV seropositive individuals or genetically modified stem cells. The invention broadly relates to the culture of cultured thymic epithelial fragments and provides procedures for verifying true immunocompetence of the resulting T cells and for analyzing the effects of various compounds on the differentiation process. The invention also comprises several novel applications for utilizing the procedure of the invention, including grafting fortified cultured thymic epithelial fragments and infusing immunocompetent T cells into patients with compromised immune systems.
REFERENCES:
Margaret Ruiz, Stanford Roodman, John Bouhasin, Alan Knutson, T Cell Differentiation/Maturation of CD34+ Stem Cell from HIV-Seropositive Hemophiliacs in Cultured Thymic Epithelial Fragments, Stem Cells 14, 132-145 (1996).
M. Rosenzweig, D.F. Marks, H. Zhu, D. Hempel, K.G. Mansfield, P.K. Sehgal, S. Kalams, D.T. Scadden, and R.P. Johnson, In Vitro T Lymphopoiesis of Human and Rhesus CD34+ Progenitor Cells, Blood 87, No. 10, 4040-4048 (1996).
Geir Erland Tjonnfjord, O.P. Veiby, R, Steen, & T. Egeland, T Lymphocyte Differentiation In Vitro from Adult Human Prethymic CD34+ Bone Marrow Cells, J. Exp. Med. 177, 1531-1539 (1993).
Andrew R. Freedman, H. Zhu, J.D. Levine, S. Kalams, & D.T. Scadden, Generation of human T lymphocytes from bone marrow CD34+ cells in vitro, Nature Medicine 2, No. 1, 46-51 (1996).
Michael Rosenzweig, E.M. Bunting, R.L. Damico, D.P. Clark, & G.N. Gaulton, Human Neonatal Thymic Organ Culture: An ex vivo Model of Thymocyte Ontogeny and HIV-1 Infection, Pathobiology 62, 245-251 (1994).
John M. Dwyer, C. C. Wood, J. McNamara, B. Kindler, Transplantation of Thymic Tissue into Patients with AIDS, Arc Intern Med. vol. 147, 513-517 (1987).
Margaret E. Ruiz, J. Freeman, J.D. Bouhasin, A.P. Knutsen, M.J.C. Hendrix, Arrest of In Vitro T Cell Differentiation of Normal Bone Marrow-Derived CD34+ Stem Cells with Thymic Epithelial Fragments from Children with AIDS, Stem Cells 14, 533-547 (1996).
Alan P. Knutsen, S.T. Roodman, M.E. Ruiz, K.R. Mueller, J.D. Bouhasin, Development of a Method of Thymocyte Differentiation of Bone Marrow-Enriched CD34+CD38- Cells in Postnatal Allogeneic Cultured Thymic Epithelia to Evaluate Immunodeficiency Disorders, Stem Cells 14, 702-713 (1996).
Bruno Peault, I.L. Weissman, C. Baum, J.M. McCune, A. Tsukamoto, Lymphoid Reconstitution of the Human Fetal thymus in SCID Mice with CD.div.+ Precursor Cells, J. Exp. Med. The Rockefeller University Press vol. 174, 1283-1286 (1991).
Jean-marie Dupuy, N. Gilmore, H. Goldman, C. Tsoukas, D. Pekovic, J.P. Chausseau, R. Duperval, M. Joly, L. Pelletier, Y. Thibaudeau, Thymic Epithelial Cell Transplantation in Patients with Acquired Immunodeficiency Syndrome, Thymus 17, 205-218 (1991).
Sven A. Danner, B.J. Schuurman, J.M.A. Lange, F.H.J. Gmelig Meyling, P.Th.A. Schellekes, J. Huber, L. Kater, Implantation of cultured Thymic Fragments in Patients with Acquired Immunodeficiency Syndrome, Arch Intern Med vol. 146, 1133-1138, (1986).
LandOfFree
T cell differentiation of CD34+ stem cells in cultured thymic ep does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with T cell differentiation of CD34+ stem cells in cultured thymic ep, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and T cell differentiation of CD34+ stem cells in cultured thymic ep will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1724762