Systems, tools and methods of assaying biological materials...

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

Reexamination Certificate

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C536S023100, C536S024300, C536S024330

Reexamination Certificate

active

07608396

ABSTRACT:
Systems, tools and methods are used to perform complex sandwich hybridization assays of biological material. The tools comprise biological solution probes having a first region for hybridizing to capture probes on a universal assay apparatus, and a second region for hybridizing to a sample target. The solution probe assembles the target to the apparatus by hybridizing the second region to the target and the first region to the capture probe. Multiple biological samples, having a plurality of targets per sample, can be multiplexed on the same universal array. The customized solution probe addresses and assembles a predetermined target-sample combination onto the array at a corresponding capture probe address location. Specificity and sensitivity of the assay are provided by the incorporation of a modified monomer in the capture probe and a similarly modified monomer complement in the first region of the solution probe. The modified monomers preferentially hybridize with each other.

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