Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2006-05-30
2006-05-30
Kim, Young J. (Department: 1637)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C536S024300, C536S024330
Reexamination Certificate
active
07052841
ABSTRACT:
Systems, tools and methods of assaying biological material are used to perform complex sandwich hybridization assays. The tools used comprise biological solution probes that are customized for each assay. The solution probe comprises a first region for hybridizing to a probe, in a generic set of capture probes on a universal assay apparatus, and a second region for hybridizing to a target in a sample. The solution probe assembles the target to the assay apparatus by hybridizing the second region to the target and the first region to the capture probe. In array assays, one or more biological samples, having one or more targets per sample, can be multiplexed on the same universal array comprising the generic set of capture probes in an array pattern of features on the substrate. The customized solution probe addresses and assembles a predetermined target-sample combination onto the array at a corresponding capture probe address location. The systems, tools and methods have specificity and sensitivity by systematically providing a reduced likelihood of cross-hybridizations and intramolecular structures in the probes. Specificity and sensitivity of the assay are provided by the incorporation of a chemically modified monomer in the capture probe and a similarly modified monomer complement in the first region of the solution probe. The modified monomers preferentially hybridize with each other. When the probe and respective probe region are oligonucleotides, the complementary modified nucleotides have a reversed polarity relative to the polarity of the respective probe and probe region. The complementary reversed polarity nucleotides form a thermodynamically more stable hybridization to each other than a hybridization between the reversed polarity nucleotide and a complementary nucleotide whose polarity is not similarly reversed.
REFERENCES:
patent: 4716106 (1987-12-01), Chiswell
patent: 4731325 (1988-03-01), Palva et al.
patent: 4751177 (1988-06-01), Stabinsky
patent: 4868105 (1989-09-01), Urdea et al.
patent: 4894325 (1990-01-01), Englehardt et al.
patent: 5194599 (1993-03-01), Froehler
patent: 5200314 (1993-04-01), Urdea
patent: 5399676 (1995-03-01), Froehler
patent: 5437976 (1995-08-01), Utermohlen
patent: 5527899 (1996-06-01), Froehler
patent: 5556749 (1996-09-01), Mitsuhashi et al.
patent: 5604097 (1997-02-01), Brenner
patent: 5635352 (1997-06-01), Urdea et al.
patent: 5635400 (1997-06-01), Brenner
patent: 5663318 (1997-09-01), Pegg et al.
patent: 5681697 (1997-10-01), Urdea et al.
patent: 5681702 (1997-10-01), Collins et al.
patent: 5721218 (1998-02-01), Froehler
patent: 5780610 (1998-07-01), Collins et al.
patent: 5846719 (1998-12-01), Brenner et al.
patent: 5858671 (1999-01-01), Jones
patent: 5871902 (1999-02-01), Weininger et al.
patent: 5922617 (1999-07-01), Wang et al.
patent: 6083763 (2000-07-01), Balch
patent: 6110678 (2000-08-01), Weisburg et al.
patent: 6185561 (2001-02-01), Balaban et al.
patent: 6232066 (2001-05-01), Felder et al.
patent: 0799897 (1997-10-01), None
patent: WO 96/41011 (1996-12-01), None
patent: WO 97/31256 (1997-08-01), None
patent: WO 98/29736 (1998-07-01), None
Definition of PNA from Wikipedia, a free on-line encyclopedia available at http://en.wikipedia.org/wiki/PNA.
M. Koga et al., “Alternating alpha,Beta-Oligothymidylates with Alternating (3′-3′)- and 5′-5′)-Internucleotidic Phosphodiester Linkages as Models for Antisense Oligodeoxyribonucleotides”, J. Org. Chem., vol. 56, No. 12, Jun. 7, 1991, pp. 3757-3759.
M. Koga et al., “The synthesis of alternating alpha, Beta-oligodeoxyribonucleotides with alternating (3′-3′)- and (5′-5′)-internucleotic linkages as potential therapeutic agents”, Nucleic Acids Symposium Series, No. 29, pp. 3-4, 1993.
M. Koga et al., “Synthesis and Physicochemical Properties of Alternating (3′-3′)- and (5′-5′)-Internucleotidic Phosphodiester Linkages”, J. Org. Chem. 1995, vol. 60, pp. 1520-1530.
Daniel D. Shoemaker et al., “Quantitative Phenotypic Analysis of Yeast Deletion Mutants Using a Highly Parallel Molecular Bar-Coding Strategy”, Nature Genetics, vol. 17, pp. 450-456, Dec. 1996.
Agilent Technologie,s Inc.
Kim Young J.
LandOfFree
Systems, tools and methods of assaying biological materials... does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Systems, tools and methods of assaying biological materials..., we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Systems, tools and methods of assaying biological materials... will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3603583