Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid
Reexamination Certificate
2008-01-01
2008-01-01
Woitach, Joseph (Department: 1636)
Chemistry: molecular biology and microbiology
Measuring or testing process involving enzymes or...
Involving nucleic acid
C435S007210, C435S007310, C536S023100
Reexamination Certificate
active
07314712
ABSTRACT:
This disclosure provides several methods to generate nucleic acid mutations in vivo, for instance in such a way that no heterologous sequence is retained after the mutagenesis is complete. The methods employ integrative recombinant oligonucleotides (IROs). Specific examples of the described mutagenesis methods enable site-specific point mutations, deletions, and insertions. Also provided are methods that enable multiple rounds of mutation and random mutagenesis in a localized region. The described methods are applicable to any organism that has a homologous recombination system.
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Lewis Lysle Kevin
Resnick Michael A.
Storici Francesca
Joike Michele K.
Klarquist Sparkman LLP.
The United States of America as represented by the Secretary of
Woitach Joseph
LandOfFree
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