Image analysis – Applications – Biomedical applications
Reexamination Certificate
2008-05-13
2008-05-13
Johns, Andrew W. (Department: 2624)
Image analysis
Applications
Biomedical applications
C382S154000
Reexamination Certificate
active
10642447
ABSTRACT:
In accordance with preferred embodiments of the present invention, a method for imaging tissue, for example, includes the steps of mounting the tissue on a computer controlled stage of a microscope, determining volumetric imaging parameters, directing at least two photons into a region of interest, scanning the region of interest across a portion of the tissue, imaging a plurality of layers of the tissue in a plurality of volumes of the tissue in the region of interest, sectioning the portion of the tissue and imaging a second plurality of layers of the tissue in a second plurality of volumes of the tissue in the region of interest, detecting a fluorescence image of the tissue due to said excitation light; and processing three-dimensional data that is collected to create a three-dimensional image of the region of interest.
REFERENCES:
patent: 4960330 (1990-10-01), Kerschmann
patent: 5139338 (1992-08-01), Pomerantz et al.
patent: 5283433 (1994-02-01), Tsien
patent: 5523543 (1996-06-01), Hunter, Jr. et al.
patent: 5804813 (1998-09-01), Wang et al.
patent: 6366357 (2002-04-01), Svetkoff et al.
patent: 6423960 (2002-07-01), Engelhardt et al.
patent: 6438261 (2002-08-01), Moshe et al.
patent: 6496267 (2002-12-01), Takaoka
patent: 6684092 (2004-01-01), Zavislan
patent: 7003143 (2006-02-01), Hewitt et al.
patent: 7110118 (2006-09-01), Ünlü et al.
patent: 7139415 (2006-11-01), Finkbeiner
patent: 7194124 (2007-03-01), Soll et al.
patent: 7197193 (2007-03-01), Li et al.
patent: 7215469 (2007-05-01), Nakata et al.
patent: 7274446 (2007-09-01), Wolleschensky et al.
patent: 2001/0052257 (2001-12-01), Magerle
patent: 2004/0076315 (2004-04-01), Scoll et al.
patent: 2004/0125372 (2004-07-01), Walla et al.
patent: 2004/0167806 (2004-08-01), Eichhorn et al.
patent: 196 32 637 (1998-02-01), None
patent: 198 19 144 (2000-06-01), None
patent: 0 209 369 (1987-01-01), None
patent: 2000-155097 (2000-06-01), None
patent: WO 98/02851 (1998-01-01), None
patent: WO 01/14853 (2001-03-01), None
Diaspro, Alberto, et al., “Adapting a Compact Confocal Microscope System to a Two-Photon Excitation Fluorescence Imaging Architecture,” Microscopy Research and Technique, 47:196-205 (1999).
Hegedüs, Csaba, et al., “3D Reconstruction Based on Hard Tissue Microtome Cross-Section Pictures in Dentistry,” Computer Methods and Programs in Biomedicine, 63:77-84 (2000).
Kim, K. H., et al., “Three-Dimensional Image Cytometer Based on a High-Speed Two-Photon Scanning Microscope,” Proc. SPIE vol. 4262, p. 238-246, Multiphoton Microscopy in the Biomedical Sciences, Ammasi Periasamy; Peter T. So; Eds., Apr. 2001.
Kim, Ki Hean, et al., “High-Speed, Two-Photon Scanning Microscope,” Applied Optics, 38(2): 6004-6009 (Oct. 1, 1999).
Periasamy, Ammasi, et al., “An Evaluation of Two-Photon Excitation Versus Confocal and Digital Deconvolution Fluorescence Microscopy Imaging in Xenopus Morphogenesis,” Microscopy Research and Technique, 47:172-181 (1999).
Rademann, Jörg, et al., “Spatially Resolved Single Bead Analysis: Homogeneity, Diffusion, and Adsorption in Cross-Linked Polystyrene,” Chem Eur. J., 7(18):3884-3889 (2001).
Shelden, E., and Knecht, D.A., “Reconstruction and Display of Curvilenear Objects From Optical Section Data Using 3-D Curve Fitting Algorithms,” Journal of Microscopy, 191(1):97-107 (Jul. 1998).
So, Peter T.C., et al., “Two-Photon Exitation Fluorescence Microscopy,” Annu. Rev. Biomed. Eng., 02:399-429 (2000).
Bird et al., Two-photon fluorescence endoscopy with a micro-optic scanning head, Optical Society America, 2003, vol. 28, No. 17, pp. 1552-1554.
Sacconi et al., Multiphoton multifocal microscopy exploiting a diffractive optical element, Optical Society America, 2003, vol. 28, No. 20, pp. 1918-1920.
Jung et al., Multiphoton endoscopy, Optical Society America, 2003, vol. 28, No. 11, pp. 902-904.
Bird et al., Fibre-optic two-photon scanning fluorescence microscopy, The Royal Microscopical Society, Journal of Microscopy, Oct. 2002, vol. 208, Pt 1, pp. 35-48.
Helmchen et al, Enchanced two-photon excitation through optical fiber by single-mode propagation in a large core, Optical Society America, May 20, 2002, vol. 41, No. 15, pp. 2930-2934.
Andersen et al., Time multiplexed multifocal multiphoton microscope, Optical Society America, Optic Letters, 2001, vol. 26, No. 2, pp. 75-77.
Helmchen et al. A miniature head-mounted two-photon microscope: high-resolution brain imaging in freely moving animals, Neuron, 2001, Cell Press, vol. 31, pp. 903-912.
Nielsen et al., High efficiency beam splitter for multifocal multiphoton microscopy, The Royal Microscopical Society, Journal of Microscopy, 2001, vol. 201, Pt 3, pp. 368-376.
Bewersdorf et al, Multifocal multiphoton microscopy, Optical Society America, Optical Letters, 1998, vol. 23, No. 9, pp. 655-657.
Buist et al., Real time two-photon absorption microscopy using multipoint excitation, The Royal Microscopical Society, Journal of Microscopy, 1998, vol. 192, Pt 2, pp. 217-226.
Denk et al., Two-photon laser scanning fluorescence microscopy, School of Applied and Engineering Physics, Dept. of Physics, Cornell University, Ithaca NY 14853.
Webb et al., A wide-field time-domain fluorescence lifetime imaging microscope with optical sectioning, American Institute of Physics, Review of Scientific Instruments, 2002, vol. 73, No. 4, pp. 1898-1907.
Leveque-Fort et al., Time-resolved multifocal multiphoton microscopy, Biomedical Optics, SPIE, 2003, vol. 5139, pp. 173-179.
Kim et al., Usage of multi anode PMT on the multi-photon fluorescence spectroscopy and video rate microscopy, IEEE, 2002.
Bahlmann Karsten
Engelward Bevin
Hsu Lily
Huang Hayden
Kim Ki Hean
Johns Andrew W.
Massachusetts Institute of Technology
Weingarten Schurgin, Gagnebin & Lebovici LLP
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