Systems and methods for the rapid isolation of nucleic acids

Details Systems and methods for the rapid isolation of nucleic acids Systems and methods for the rapid isolation of nucleic acids

1996-01-25

2000-02-22

Smith, Lynette R. F.

Drug, bio-affecting and body treating compositions

Preparations characterized by special physical form

Particulate form

536 254, 210 27, 210 28, 210 29, 210 40, A61K 914, C07H 2100, C07H 2102, C07H 2104

Patent

active

060277505

ABSTRACT:
The present invention contemplates a system for rapidly isolating nucleic acids. The system comprises an insoluble silica matrix and a buffered aqueous salt solution containing salt at a concentration of at least 3 molar and a buffering agent at a concentration sufficient to provide a buffering capacity corresponding to that which either tris(hydroxymethyl)aminomethane or phosphate ion at a concentration of 0.1 to 1 molar would provide in the solution. Methods of using the system are also contemplated.

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Vogelstein et al, "Preparative and Analytical Purification of DNA fro Agarose," Proc. Natl. Acad. Sci, USA, 7:615-619 (1979).
Birnboim, "A Rapid Alkaline Extraction Method for the Isolation of Plasmid DNA," Meth. Enzol., 100:243-255 (1983).
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Marko et al, "A Procedure for the Large-Scale Isolation of Highly Purified Plasmid DNA Using Alkaline Extraction and Binding to Glass Powder," Anal. Biochem., 121:382-387 (1982).
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