Combinatorial chemistry technology: method – library – apparatus – Method of screening a library – By measuring the ability to specifically bind a target...
Reexamination Certificate
2008-02-19
2010-11-09
Lundgren, Jeffrey S (Department: 1639)
Combinatorial chemistry technology: method, library, apparatus
Method of screening a library
By measuring the ability to specifically bind a target...
C506S016000
Reexamination Certificate
active
07829502
ABSTRACT:
A method for identifying aptamers that bind to target molecules may include contacting an oligonucleotide library with target molecule and digesting unbound oligonucleotides with one or more endonucleases, one or more exonucleases, or one or more endonucleases in combination with one or more exonucleases. A method for identifying aptamers may further include optionally subjecting selected aptamers to one or more rounds of selection under conditions of increased stringency. A method for identifying aptamers may include yet further amplifying selected aptamers. The described methods may be performed in a screen for identifying aptamers either alone or in combination with other methods typically employed in the art for selecting aptamers (such as, e.g., SELEX). Also contemplated herein are systems and kits for accomplishing the above.
REFERENCES:
Wang (Aug. 24, 2004) Analytical Chemistry vol. 76 pp. 5605 to 5610.
Kang (Dec. 9, 2004) Proceedings of the National Academy of Sciences USA vol. 101 pp. 17611 to 17615.
Boesen Christian
Lundgren Jeffrey S
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