Systemin, an inducer of plant defense proteins, and methods of u

Details Systemin, an inducer of plant defense proteins, and methods of u Systemin, an inducer of plant defense proteins, and methods of u

1992-03-19

1995-01-03

Hill, Jr., Robert J.

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

536236, 435 6, 435 691, 4353201, 530379, C07H 1700, C12N 1500, C12P 1934, C12Q 168

Patent

active

053788192

ABSTRACT:
Systemin is an 18 amino acid peptide hormone that induces expression of defense genes in plants wounded mechanically or by predators including herbivores, insects, bacteria and viruses. The precursor for systemin is encoded as a 200 amino acid prosystemin molecule that has the systemin peptide sequence located near the carboxy-terminus. Both a 951 bp cDNA for prosystemin and 4526 bp genomic DNA were cloned and the organization of the gene was determined. Transgenic plants constructed with antisense prosystemin DNA fail to mount a defensive response to wounding. Transgenic plants constructed with increased copy number of prosystemin genes exhibit increase resistance to wounding. A tomato systemin polypeptide has an amino acid sequence NH.sub.3 --AVQSKPPSKRDPPKMQTD--COO--.

REFERENCES:
patent: 4394443 (1983-07-01), Weissman et al.
Pearce et al. (23 Aug. 1991) Science 253: 895-898.
Ryan, C. A., "Protease Inhibitors in Plants: Genes for Improving Defenses Against Insects and Pathogens," Ann. Rev. Phytopathol. 28, 425-449 (1990).
Bowles, D. J., "Defense-Related Proteins in Higher Plants," Ann Rev. Biochem. 59, 873-907 (1990).
Chessin, M. and A. E. Zipf, "Alarm Systems in Higher Plants," The Botanical Review 56, 193-235 (1990).
Dreyer, D. L. and B. C. Campbell, "Chemical basis of host-plant resistance to aphids," Plant, Cell and Environ. 10, 353-361 (1987).
Green, T. R. and C. A. Ryan, "Wound-induced Proteinase Inhibitor in Plant Leaves: A Possible Defense Mechanism against Insects," Science 175, 776-777 (1972).
Ryan, C. A., "Proteinase inhibitor in plant leaves: A biochemical model for pest-induced natural plant protection," TIBS 3, No. 7, 148-150 (1978).
Hilder, V. A., A. M. R. Gatehouse, S. E. Sheerman, R. F. Barker, and D. Boulter, "A novel mechanism of insect resistance engineered into tobacco," Nature 330, 160-163 (1987).
Johnson, R., J. Narvaez, G. An, and C. A. Ryan, "Expression of proteinase inhibitors I and II mRNAs in transgenic tobacco plants: Effects on natural defense against Manduca sexta larvae," Proc. Natl. Acad. Sci. U.S.A. 86, 9871-9875 (1989).
Graham, J. S., G. Hall, G. Pearce, and C. A. Ryan, "Regulation of synthesis of proteinase inhibitors I and II mRNAS in leaves of wounded tomato plants," Planta 169, 399-405 (1986).
Graham, J. S., G. Pearce, J. Merryweather, K. Titani, L. Ericsson, and C. A. Ryan, "Wound-induced Proteinase Inhibitors from Tomato Leaves," J. Biol. Chem. 260, No. 11, 6555-6560 and 6561-6564, (1985).
Ryan, C. A., "An Inducible Protein in Potato and Tomato Leaflets," Plant Physiol. 43, 1880-1881 (1968).
Brown, W. E. and C. A. Ryan, "Isolation and Characterization of a Wound-Induced Trypsin Inhibitor from Alfalfa Leaves," Biochemistry 23, 3418-3422 (1984).
Brown, W. E., K. Takio, K. Titani, and C. A. Ryan, "Wound-Induced Trypsin Inhibitor in Alfalfa Leaves: Identity as a Member of the Bowman-Birk Inhibitor Family," Biochemistry 24, 2105-2108 (1985).
Roby, D., A. Toppan, and M. T. Esquerre-Tugaye, "Cell surfaces in plant micro-organism interactions. VIII. Increased proteinase inhibitor activity in melon plants in response to infection by Colletotrichum lagenarium or to treatment with an elicitor fraction from this fungus," Physiol. Mol. Pl. Pathol. 30, 453-460 (1987).
Bradshaw, H. D., J. B. Hollick, T. J. Parsons, H. R. G. Clarke, and M. P. Gordon, "Systemically wound-responsive genes in poplar trees encode proteins similar to sweet potato sporamins and legume Kunitz trypsin inhibitors," Plant Mol. Biol. 14, 51-59 (1989).
Kuc, J. and C. Preisig, "Fungal Regulation of Disease Resistance Mechanisms in Plants," Mycologia 76, 767-784 (1984).
Kopp, M. J. Rouster, B. Fritig, A. Darvill, and P. Albersheim, "Host-Pathogen Interactions," Plant Physiol. 90, 208-216 (1990).
Hammond-Kosack, K. E., H. J. Atkinson and Dianna J. Bowles, "Systemic accumulation of novel proteins in the apoplast of the leaves of potato plants following root invasion by the cyst-nematode Globodera rostochiensis," Physiol. Mol. Plant Path. 35: 395-506 (1989).
Ryan, C. A. and E. E. Farmer, "Oligosaccharide Signals in Plants: A Current Assessment," Annu. Rev. Plant. Physiol. Mol. Bio. 42, 651-674 (1991).
Farmer, E. E. and C. A. Ryan, "Interplant communication: Airoborne methyl jasmonate induces synthesis of proteinase inhibitors in plant leaves," Proc. Natl. Acad. Sci. U.S.A. 87, 7713-7716 (1990).
Pena-Cortes, H., J. J. Sanchez-Serrano, R. Mertens, L. Willmitzer and S. Prat, "Abscisic acid is involved in the wound-induced expression of the proteinase inhibitor II gene in potato and tomato," Proc. Natl. Acad. Sci. U.S.A. 86, 9851-9855 (1989).
Davies, E., "Action potentials as multifunctional signals in plants: a unigying hypothesis to explain apparently disparate wound responses," Plant, Cell and Environ. 10, 623-631 (1987).
Thain, J. F., H. M. Doherty, D. J. Bowles and D. C. Wildon, "Oligosaccharides that induce proteinase inhibitor activity in tomato plants cause depolarization of tomato leaf cells," Plant, Cell and Environ. 13, 569-574 (1990).
Pearch, G. D. Strydom, S. Johnson and C. A. Ryan, "A Polypeptide from Tomato Leaves induces Wound-Inducible Proteinase Inhibitor Proteins," Science 253, 895-898 (1991).
McGurl, G. and C. A. Ryan, "The Organization of the Prosystemin Gene," Plant Molecular Biology, submitted.
Lutcke, H. A., K. C. Chow, F. S. Mickel, K. A. Moss, H. F. Kern and G. A. Scheele, "Selection of AUG initiation codons differs in plants and animals," EMBO Journal 6, 43-48 (1987).
Harris, R. B., "Processing of Pro-hormone Precursor Proteins," Arch. Biochem. Biophys. 275, No. 2, 315-333 (1989).
Douglass, J., O. Civelli and E. Herbert, "Polyprotein Gene Expression: Generation of Diversity of Neuroendocrine Peptides," Ann. Rev. Biochem. 53, 665-715 (1984).
Jung, L. J. and R. H. Schefler, "Peptide Processing and Targeting in the Neuronal Secretory Pathway," Science 251, 1330-1335 (1991).
Ryan, C. A., "Quantitative Determination of Soluble Cellular Proteins by Radial Diffusion in Agar Gels Containing Antibodies," Anal. Biochem. 19, 434-440 (1967).
Trautman, R., K. M. Cowan, G. G. Wagner, "Data Processing for Radial Immunodiffusion," Immunochemistry 8, 901-916 (1971).
Hopp, T. P. and K. R. Woods, "Prediction of protein antigenic determinants from amino acid sequences," Proc. Nat. Acad. Sci. 78, 3824-3826 (1981).
Schechter, I. and A. Berger, "On the size of the active site in proteases. I. Papain," Biochem. Biophys. Res. Commun. 27, 157-162 (1967).
Rogers, S. O. and A. J. Bendich, "Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues," Plant Mol. Biol. 5, 69-76 (1985).
Baydoun, E. A.-H. and S. C. Fry, "The immobility of pectic substances in injured tomato leaves and its bearing on the identity of the wound hormone," Planta 165, 269-276 (1985).
Smith, P. K., R. I. Krohn, G. T. Hermanson, A. K. Mallia, F. H. Gartner, M. D. Provenzano, E. K. Fujimoto, N. M. Goeke, B. J. Olson and D. C. Klenk, "Measurement of Protein Using Bicinchoninic Acid," Anal. Biochem. 150, 76-85 (1985).
Strydorn, D. J., J. Wade Harper and Roy R. Lobb, "Amino Acid Sequence of Bovine Brain Derived class 1 Heparin-Binding Growth Factor," Biochemistry 25, 945-951 (1985).
Bidlingmeyer, B. A., S. A. Cohen and T. L. Tarvine, "Rapid Analysis of Amino Acids Using Pre-Column Derivatization," J. Chromatogr. 336, 93-104 (1984).
King, R. W. and J. A. D. Zeevaart, "Enhancement of Phloem Exudation from Cut Petioles by Chelating Agents," Plant Physiol. 53, 96-103 (1974).

Affiliated with

Also associated with

Rating

Systemin, an inducer of plant defense proteins, and methods of u does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Systemin, an inducer of plant defense proteins, and methods of u, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Systemin, an inducer of plant defense proteins, and methods of u will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-2212497