Chemistry: molecular biology and microbiology – Vector – per se
Patent
1997-07-09
2000-02-08
Patterson, Jr., Charles L.
Chemistry: molecular biology and microbiology
Vector, per se
536 236, 514 12, 514 13, C12N 1582, C12N 1529
Patent
active
060227397
ABSTRACT:
Systemin, an 18 amino acid peptide hormone and first polypeptide hormone found in plants, induces expression of defense genes in plants wounded mechanically or by predators including herbivores, insects, bacteria and viruses. The precursor for systemin is encoded as a 200 amino acid prosystemin molecule that has the systemin peptide sequence located near the carboxyl-terminus. Both a 951 bp cDNA for prosystemin and 4526 bp genomic DNA were cloned and the organization of the gene was determined. Transgenic plants constructed with antisense prosystemin DNA fail to mount a defensive response to wounding. Transgenic plants constructed with increased copy number of prosystemin genes exhibit increased resistance to wounding. Insect larval that feed on transgenic plants constructed with increased copy number of prosystemin genes exhibit decreased growth weight compared to larval that feed on wild type plants.
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McGurl Barry F.
Pearce Gregory L.
Ryan Clarence A.
Patterson Jr. Charles L.
Washington State University Research Foundation, Inc.
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