Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai
Patent
1994-09-19
1999-03-16
Patterson, Jr., Charles L.
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Peptide containing doai
514 13, 530379, A61K 3804, A61K 3816, A61K 14415
Patent
active
058830766
ABSTRACT:
Systemin, an 18 amino acid peptide hormone and first polypeptide hormone found in plants, induces expression of defense genes in plants wounded mechanically or by predators including herbivores, insects, bacteria and viruses. The precursor for systemin is encoded as a 200 amino acid prosystemin molecule that has the systemin peptide sequence located near the carboxyl-terminus. Both a 951 bp cDNA for prosystemin and 4526 bp genomic DNA were cloned and the organization of the gene was determined. Transgenic plants constructed with antisense prosystemin DNA fail to mount a defensive response to wounding. Transgenic plants constructed with increased copy number of prosystemin genes exhibit increased resistance to wounding. Insect larval that feed on transgenic plants constructed with increased copy number of prosystemin genes exhibit decreased growth weight compared to larval that feed on wild type plants. A tomato systemin polypeptide has an amino acid sequence NH.sub.3 -AVQSKPPSKRDPPKMQTD-COO-.
REFERENCES:
patent: 4394443 (1983-07-01), Weismann et al.
patent: 5378819 (1995-01-01), Ryan et al.
Rochaix, J-D, "Plant Molecular Biology--Moving Towards Application," Trends Biotech. 10:78-79 (1992).
Chasan, R., "Plant Molecular Biology Blossoms in the Desert," Plant Cell 3:1255-1262 (1991).
Culver, J.N. et al., "Tobacco Mosaic Virus Elicitor Coat Protein Genes Produce a Hypersensitive Phenotype in Transgenic Nicotiana Sylvestris Plants," Mol. Plant-Microbe Inter. 4:458-463 (1991).
Ryan, C.A. et al., "Systemin A. Polypeptide Signal for Proteinase Inhibitor Gene Expression in Plants," Dev. Plant Pathol. 2:196-201 (1992).
Baydoun, E.A.-H. et al., "The Immobility of Pectic Substances in Injured Tomato Leaves and its Bearing on the Identity of the Wound Hormone," Planta 165:269-276 (1985).
Bidlingmeyer, B.A. et al., "Rapid Analysis of Amino Acids Using Pre-Column Derivatization," J. Chromatogr. 336:93-104 (1984).
Bowles, D.J., "Defense-related Proteins in Higher Plants," Ann. Rev. Biochem. 59:873-907 (1990).
Bradshaw, H.D. et al., "Systemically wound-responsive genes in poplar trees encode proteins similar to sweet potato sporamins and legume Kunitz trypsin inhibitors," Plant Mol. Biol. 14, 51-59 (1989).
Brown, W.E. et al., "Isolation and Characterization of a Wound-Induced Trypsin Inhibitor from Alfalfa Leaves," Biochemistry 23:3418-3422 (1984).
Brown, W.E. et al., "Wound-Induced Trypsin Inhibitor from Alfalfa Leaves: Identity as a Member of the Bowman-Birk Inhibitor Family," Biochemistry 24:2105-2108 (1984).
Chessin, M. et al., "Alarm Systems in Higher Plants," The Botanical Review 56:193-235 (1990).
Davies, E., "Action Potentials as Multifunctional Signals in Plants: A Unifying Hypothesis to Explain Apparently Disparate Wound Responses," Plant, Cell and Environ. 10:623-631 (1987).
Douglass, J. et al., "Polyprotein Gene Expression: Generation of Diversity of Neuroendocrine Peptides," Ann. Rev. Biochem. 53:665-715 (1984).
Dreyer, D.L. et al., "Chemical Basis of Host-Plant Resistance to Aphids," Plant, Cell and Environ. 10:353-361 (1987).
Farmer, E.E. et al., "Interplant Communication: Airborne Methyl Jasmonate Induces Synthesis of Proteinase Inhibitors in Plant Leaves," Proc. Natl. Acad. Sci. U.S.A. 87:7713-7716 (1990).
Graham, J.S. et al., "Regulation of Syntheses of Proteinase Inhibitors I and II mRNAs in Leaves of Wounded Tomato Plants," Planta 169:399-405 (1986).
Graham, J.S. et al., "Wound-Induced Proteinase Inhibitors from Tomato Leaves," J. Biol. Chem. 260:6555-6560 (1985).
Green, T.R. et al., "Wound-Induced Proteinase Inhibitor in Plant Leaves: A Possible Defense Mechanism against Insects," Science 175:776-777 (1972).
Hammond-Kosack, K.E. et al., "Systemic Accumulation of Novel Proteins in the Apoplast of the Leaves of Potato Plants following Root Invasion by the Cyst-Nematode Globodera rostochiensis," Physiol. Mol. Plant Path. 35:495-506 (1989).
Harris, R.B., "Processing of Pro-hormone Precursor Proteins," Arch. Biochem. Biophys. 275:315-333 (1989).
Hilder, V.A. et al., "A Novel Mechanism of Insect Resistance Engineered into Tobacco," Nature 330:160-163 (1987).
Hopp, T.P. et al., "Prediction of Protein Antigenic Determinants from Animo Acid Sequences," PNAS (USA) 78:3824-3826 (1981).
Johnson, R. et al., "Expression of Proteinase Inhibitors I and II mRNAs in Transgenic Tobacco Plants: Effects on Natural Defense Against Manduca sexta Larvae," PNAS (USA) 86:9871-9875 (1989).
Jung, L.J. et al., "Peptide Processing and Targeting in the Neuronal Secretory Pathway," Science 251:1330-1335 (1991).
King, R.W. et al., "Enhancement of Phtoem Exudation from Cut Petioles by Chelating Agents," Plant Physiol. 53:96-103 (1974).
Kopp, M.J. et al, "Host-Pathogen Interactions," Plant Physiol. 90:208-216 (1990).
Kuc, J. et al., "Fungal Regulation of Disease Resistance Mechanisms in Plants," 76:767-784 (1984).
Lutcke, H.A. et al., "Selection of AUG Initiation Codons Differs in Plants and Animals," EMBO J. 6:43-48 (1987).
McGurl, B. et al., "The Organization of the Prosystemin Gene," Plant Mol. Biol., submitted (no date).
Pearce, G. et al., "A Polypeptide from Tomato Leaves Induces Wound-Inducible Proteinase Inhibitor Proteins," Science 253-895-898 (1991).
Pena-Cortes, H. et al., "Abscisic Acid is Involved in the Wound-Induced Expression of the Proteinase Inhibitor II Gene in Potato and Tomato," PNAS (USA) 86:9851-9855 (1989).
Roby, D. et al., "Cell Surfaces in Plant Micro-Organism Interactions. VIII. Increased Proteinase Inhibitor Activity in Melon Plants in Response to Infection by Colletotrichum lagenarium or to Treatment with an Elicitor Fraction from this Fungus," Physiol. Mol. Pl. Pathol. 30:453-460 (1987).
Rogers, S.O. et al., "Extraction of DNA from Milligram Amounts of Fresh, Herbarium and Mummified Plant Tissues," Plant Mol. Biol. 5:69-76 (1985).
Ryan, C.A. "Protease Inhibitors in Plants: Genes for Improviing Defenses Against Insects and Pathogens," Ann. Rev. Phytopathol. 28:425-449 (1990).
Ryan, C.A., "Proteinase Inhibitors in Plant Leaves: A Biochemical Model for Pest-Induced Natural Plant Protection," TIBS 3:148-150 (1978).
Ryan, C.A. "An Inducible Protein in Potato and Tomato Leaflets," Plant Physiol. 43: 1880-1881 (1968).
Ryan, C.A. et al., "Oligosaccharide Signals in Plants: A Current Assessment," Annu. Rev. Plant. Physiol. Mol. Bio. 42:651-674 (1991).
Ryan, C.A., "Quantitative Determination of Soluble Cellular Proteins by Radial Diffusion in Agar Gels Containing Antibodies," Anal. Biochem. 19:434-440 (1967).
Schechter, I. et al., "On the Size of the Active Site in Proteases. I. Papain," Biochem. Biophys. Res. Commun. 27:157-162 (1967).
Smith, P.K. et al., "Measurement of Protein Using Bicinchoninic Acid," Anal. Biochem. 150:76-85 (1985).
Strydorn, D.J. et al., "Amino Acid Sequence of Bovine Brain Derived Class 1 Heparin-Binding Growth Factor," Biochemistry 25:945-951 (1985).
Thain, J.F. et al., "Oligosaccharides that Induce Proteinase Inhibitor Activity in Tomato Plants Cause Depolarization of Tomato Leaf Cells," Plant Cell and Environ. 13:569-574 (1990).
Trautman, R. et al., "Data Processing for Radial Immunodiffusion," Immunochemistry 8:901-916 (1971).
McGurl Barry F.
Pearce Gregory L.
Ryan Clarence A.
Patterson Jr. Charles L.
Washington State University Research Foundation, Inc.
LandOfFree
Systemin does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Systemin, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Systemin will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-817799