Systematic evolution of ligands by exponential enrichment:...

Details Systematic evolution of ligands by exponential enrichment:... Systematic evolution of ligands by exponential enrichment:...

2007-02-13

2007-02-13

Schultz, James (Department: 1635)

Organic compounds -- part of the class 532-570 series

Organic compounds

Carbohydrates or derivatives

C435S006120, C435S091200, C536S025400

Reexamination Certificate

active

10263456

ABSTRACT:
A method is described for generating blended nucleic acid ligands containing non-nucleic acid functional units. Specifically, a SELEX identified RNA ligand to the integrin gpIIbIIIa is conjugated to the peptide Gly-Arg-Gly-Asp-Thr-Pro (SEQ ID NO:1). This blended RNA ligand inhibits the biological activity of gpIIbIIIa with high specificity. Also described is a single-stranded DNA ligand to elastase coupled to N-methoxysuccinyl-Ala-Ala-Pro-Val-chloromethyl ketone (SEQ ID NO:2). This elastase blended nucleic acid ligand inhibits the biological activity of elastase.

REFERENCES:
patent: 3755295 (1973-08-01), Verheyden et al.
patent: 5118672 (1992-06-01), Shinazi et al.
patent: 5270163 (1993-12-01), Gold et al.
patent: 5683867 (1997-11-01), Biesecker et al.
patent: 5705337 (1998-01-01), Gold et al.
patent: 5723323 (1998-03-01), Kauffman et al.
patent: 5763595 (1998-06-01), Gold et al.
patent: 5962219 (1999-10-01), Gold et al.
patent: 5998142 (1999-12-01), Gold et al.
patent: 6011020 (2000-01-01), Gold et al.
patent: 6083696 (2000-07-01), Biesecker et al.
patent: 6147204 (2000-11-01), Gold et al.
patent: 6300074 (2001-10-01), Gold et al.
patent: 6465188 (2002-10-01), Gold et al.
patent: 6465189 (2002-10-01), Gold et al.
patent: 2003/0125263 (2003-07-01), Gold et al.
patent: 2003/0186231 (2003-10-01), Gold et al.
patent: 2005/0164974 (2005-07-01), Gold et al.
patent: 2 183 661 (1987-06-01), None
patent: WO 89/06694 (1989-07-01), None
patent: WO 91/14696 (1991-10-01), None
patent: WO 91/19813 (1991-12-01), None
patent: WO 92/14843 (1992-09-01), None
patent: WO 93/04204 (1993-03-01), None
patent: WO 94/08050 (1994-04-01), None
patent: WO 95/16788 (1995-06-01), None
patent: WO 96/27605 (1996-09-01), None
patent: WO 96/34876 (1996-11-01), None
Barrett (1978) Agents and Actions 8:11.
Bonney (1989) J. Cell. Biochem. 39:47.
Dewald et al. (1975) J. Exp. Med. 141:709.
Ellington & Szostak (1990) Abstract of papers presented at the 1990 meeting on RNA Processing, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York, p. 84.
Frojmovic et al. (1991) Blood 78:369.
Hemmi et al. (1985) Biochemistry 24:1841.
Joyce (1989) Gene 82:83.
Joyce & Inoue (1989) Nucleic Acids Research 17:711.
Kaplan et al. (1973) J. Lab. Clin. Med. 82:349.
Kinzler & Vogelstein (1989) Nucleic Acids Research 17:3645.
Kramer et al. (1974) J. Mol. Biol. 89:719.
Levisohn & Spiegelman (1969) Proc. Natl. Acad. Sci. USA 63:805.
Levisohn & Spiegelman (1968) Proc. Natl. Acad. Sci. USA 60:866.
Loftus et al. (1990) Science 249:915.
Oleksyszyn and Powers (1989) Biochem. and Biophys. Res. Comm. 161:143.
Oliphant et al. (1989) Mol. Cell. Biol. 9:2944.
Oliphant et al. (1986) Gene 44:177.
Oliphant & Struhl (1988) Nucleic Acids Research 16:7673.
Oliphant & Struhl (1987) Methods in Enzymology 155:568.
Phillips et al. (1988) Blood 71:831.
Robertson & Joyce (1990) Nature 344:467.
Sanders & Moore (1983) An. Rev. Respir. Dis. 127:554.
Szostak, Structure and Activity of Ribozymes inRedesigning the Molecules of Life(S.A. Benner ed.) Spring-Verlage Berline Heidelberg, pp. 87-113 (1988).
Thiesen & Back (1990) Nucleic Acids Research 18:3203.
Ware & Heistad (1993) N. Eng. J. Med. 328:628.
Weiland et al. (1986) Am. Rev. Respir. Dis. 133:218.
Aurup et al. (1992) Biochem. 31:9636.
Bock et al. (1992) Nature 355:564-566.
Chen et al. (1977) Biochem. 16:3310.
Jakeman et al. (1992) J. Clin. Invest. 89:244.
Lestienne et al. (1983) Biochimie 65:49-52.
Matthews et al. (1988) Anal. Biochem. 169:1-25.
Oleksyszyn et al. (1991) Biochemistry 30:485-493.
Peet et al. (1990) J. Med Chem 33:394-407.
Pieken et al. (1991) Science 253:314-317.
Talbot et al. (1990) Nucl. Acids Res. 18:3521.
Tuerk et al. (1990) Science 249:505-510.
Tuerk et al. (1992) Proc. Natl. Acad. Science 89(15):6988-92.
Verheyden et al. (1971) J. Org. Chem. 36:250.
Weintraub (1973) Cold Spring Harbour Symp. Quant. Biol. 38:247.
Milligan et al. (1993) J., Medicinal Chem. 36:1923-37.

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