System to detect protein-protein interactions

Details System to detect protein-protein interactions System to detect protein-protein interactions

1994-02-01

1995-11-21

Parr, Margaret

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

4353201, 536 237, 536 234, 935 79, 935 80, C12Q 168

Patent

active

054686148

ABSTRACT:
A method and kit are provided for detecting the interaction between a first test protein and a second test protein, in vivo, using reconstitution of the activity of a transcriptional activator. This reconstitution makes use of chimeric genes which express hybrid proteins. Two types of hybrid proteins are prepared. The first hybrid contains the DNA-binding domain of a transcriptional activator fused to the first test protein. The second hybrid protein contains a transcriptional activation domain fused to the second test protein. If the two test proteins are able to interact, they bring into close proximity the two domains of the transcriptional activator. This proximity is sufficient to cause transcription, which can be detected by the activity of a marker gene which contains a binding site for the DNA-binding domain.

REFERENCES:
patent: 5283173 (1994-02-01), Fields
Keegan et al., Science, 231, 699-704 (1986).
McKnight et al., Proc. Nat'l. Acad. Sci. USA, 84, 7061-7065 (1987).
Curran et al., Cell, 55, 395-397 (1988).
Johnston, Microbiol. Rev., 51, 458-476 (1987).
Brent and Ptashne, Cell, 43, 729-736 (1985).
Ma and Ptashne, Cell, 51, 113-119 (1987).
Hope and Struhl, Cell, 46, 885-894 (1986).
Thukral et al., Mol. Cell. Biol., 9, 2360-2369.
Kumar et al., Cell, 51, 941-951 (1987).
Celenza and Carlson, Science, 233, 1175-1180 (1986).
Celenza and Carlson, Mol. Cell. Biol., 9, 5034-5044 (1989).
Celenza, Eng, and Carlson, Mol. Cellular Biol., 4, 5045-5054 (1989).
Laughon et al., Mol. Cell. Biol., 4, 260-267 (1984).
Ma and Ptashne, Cell, 48, 847-853 (1987).
Gill and Ptashne, Cell, 51, 121-126 (1987).
Silver et al., Proc. Nat'l. Acad. Sci. USA, 81, 5951-5955 (1984).
Broach et al., Gene, 8, 121-123 (1979).
Ma and Ptashne, Cell, 55, 443-446 (1988).
Wittekind et al., Mol. Cell. Biol., 8, 3997-4008 (1988).
Hope et al., Nature, 333, 635-640 (1988).
Fields and Song, Nature, 340, 245-248 (1989).
Kakidani and Ptashne, Cell, 52, 161-167 (1988).
Ma et al., Nature, 334, 631-633 (1988).
Fischer et al., Nature, 332 853-856 (1988).
Chien et al. Proc. Natl. Acad. Sci., vol. 88, 9578-9582 (1991).
1988 Stratagere Catalog p. 39.
Stryer, "Biochemistry," published 1981 by W. H. Freeman and Co., pp. 120-123.

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