System to detect protein-protein interactions

Details System to detect protein-protein interactions System to detect protein-protein interactions

1995-06-07

1997-09-16

Elliott, George G.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

435 691, 4351723, 530350, C12Q 168

Patent

active

056679734

ABSTRACT:
A method is provided for detecting the interaction between a first test protein and a second test protein, in vivo, using reconstitution of the activity of a transcriptional activator. This reconstitution makes use of chimeric genes which express hybrid proteins. Two types of hybrid proteins are prepared. The first hybrid contains the DNA-binding domain of a transcriptional activator fused to the first test protein. The second hybrid protein contains a transcriptional activation domain fused to the second test protein. If the two test proteins are able to interact, they bring into close proximity the two domains of the transcriptional activator. This proximity is sufficient to cause transcription, which can be detected by the activity of a marker gene which contains a binding site for the DNA-binding domain.

REFERENCES:
patent: 5283173 (1994-02-01), Fields et al.
Chien et al., Proc. Natl. Acad. Sci., 88(21):9578-9582 Nov. 1, 1991.
Stryer, "Biochemistry," published 1981 by W. H. Freeman and Co., pp. 120-123.

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