System of reactants involving an apo-enzyme useful in immunologi

Details System of reactants involving an apo-enzyme useful in immunologi System of reactants involving an apo-enzyme useful in immunologi

1988-01-06

1990-10-02

Kepplinger, Esther L.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

435 15, 435 21, 435 24, 435 25, 436501, 436537, G01N 33535, G01N 33542

Patent

active

049606930

ABSTRACT:
A quick and simple immunoassay technique applicable to the determination of large molecules like proteins, polynucleotides and others involves labelling competitive immunospecies with an enzyme as signal generator. This enzyme can be converted to the corresponding apo-enzyme form and its regeneration, upon addition of a suitable co-factor, is inhibited by complexation when the labelled specie is reacted with its immunopartner. The degree of complexation is therefore ascertained by measuring the extent of regenerated activity.

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