Chemistry: molecular biology and microbiology – Virus or bacteriophage – except for viral vector or...
Patent
1998-06-22
1999-12-28
Brusca, John S.
Chemistry: molecular biology and microbiology
Virus or bacteriophage, except for viral vector or...
4353201, 435325, 536 231, 536 2372, 424 936, C12N 700, C12N 510, C12N 701, C12N 1511, C12N 1540
Patent
active
060080353
ABSTRACT:
The present invention provides a method of delivering immunogenic or therapeutic proteins to bone marrow cells using alphavirus vectors. The alphavirus vectors disclosed herein target specifically to bone marrow tissue, and viral genomes persist in bone marrow for at least three months post-infection. No or very low levels of virus were detected in quadricep, brain, and sera of treated animals. The sequence of a consensus Sindbis cDNA clone, pTR339, and infectious RNA transcripts, infectious virus particles, and pharmaceutical formulations derived therefrom are also disclosed. The sequence of the genomic RNA of the Girdwood S.A. virus, and cDNA clones, infectious RNA transcripts, infectious virus particles, and pharmaceutical formulations derived therefrom are also disclosed.
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Davis Nancy L.
Johnston Robert E.
Simpson Dennis A.
Brusca John S.
The University of North Carolina at Chapel Hill
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