System for simultaneously conducting multiple ligand binding ass

Details System for simultaneously conducting multiple ligand binding ass System for simultaneously conducting multiple ligand binding ass

1997-09-04

2000-08-29

Swartz, Rodney P.

Chemistry: analytical and immunological testing

Involving an insoluble carrier for immobilizing immunochemicals

Carrier is inorganic

356244, 356246, 356317, 356318, 385 12, 385 13, 385244, 422 57, 422 8205, 422 8208, 422 8211, 435 4, 436501, 436518, G01N 2101, G01N 2164, G01N 33552, G02B 600

Patent

active

061107492

ABSTRACT:
A system for simultaneously conducting multiple ligand assays on a sample potentially containing target analytes uses as a detector a waveguide having a planar surface with a plurality of probes of known recognition to the target analytes thereon. The probes are in discrete areas on the waveguide. A sample containing target analyte is treated with a light-responsive compound such that it binds to the target analyte to form a conjugate and the conjugate is applied to the probes on the waveguide. A laser light is passed into the planar surface of the waveguide at a plurality of different locations, by causing relative movement between the waveguide and the laser light, so that evanescent waves radiate from the waveguide. Where conjugate has attached to a probe, there is emission of light different from that emitted by a probe without conjugate attached thereto.

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