System for sample detection with compensation for difference in

Chemistry: analytical and immunological testing – Peptide – protein or amino acid – Amino acid or sequencing procedure

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436 53, 436 86, 436 89, 436164, 436172, 435 6, 356344, 204452, 204461, G01N 2100, G01N 2164, G01N 3508, C12Q 168

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active

055039944

ABSTRACT:
In many separation techniques, such as field flow fractionation, liquid chromatography and electro-phoresis, chemical species form bands that migrate at different velocities. If the data-digitization rate and excitation intensity are both set to be optimal for the fastest migrating band, to compensate for different band velocities, both the data-digitization rate and the excitation intensity are decreased as a function of time by a factor equal to the migration time of the fastest migrating band to the separation time.

REFERENCES:
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patent: 4769523 (1988-09-01), Tanimoto et al.
Kambara et al. (1992) Electrophoresis, vol. 13, pp. 542-546.
L. M. Smith et al., Nature, 321:674-679 (1986).
L. R. Middendorf et al., Electrophoresis, 13:487-494 (1992).
S. Pentoney et al., Electrophoresis, 13:467-474 (1992).
X. Huang et al., "Analysis of Facotrs Causing Peak Broadening in Capillary Zone Electrophoresis,"Journ. of Chrom., 480:95-110 (1989).
J. C. White et al., "Photostability Studies of Phycobiliprotein Fluorescent Labels,"Anal. Biochem., 161:442-452 (1987).

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