System for release of proteins from microbe cells

Details System for release of proteins from microbe cells System for release of proteins from microbe cells

1987-02-06

1990-08-14

Warren, Charles E.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

435 691, 435 697, 435 698, 435 712, 43525233, 435259, 435320, 435849, 935 47, 935 48, C12N 120, C12N 106, C12N 1500, C12P 2100

Patent

active

049487353

ABSTRACT:
The invention concerns novel and useful mutant microbes which are capable of releasing substantial amounts of any of several periplasmic or recombinant proteins into the culture medium when carrying an expressed Kil gene. Though E. coli are exemplified, the invention is broadly applicable to the making of mutants of other microbes, for example, Salmonella, Klebsiella, and Rhizobium. A key feature of the invention is the use of a novel selection procedure employing a plasmid comprising the kil gene.

REFERENCES:
Kudo et al., 1983, J. of Bacteriology 156: 949-951.
Pugsley et al., 1983, J of Bacteriology 156: 109-114.
Pugsley et al., 1984, Embo J. 3: 2393-2397.
Cole et al., 1985, MGG 198: 465-472.
Luirink et al., (Mar. 1986) J of Gen. Microbiology 132: 825-834.
Suit et al., 1988, J of Bacteriol. 170: 4963-4966.
Pugsley, A. P., and M. Schwartz (1984) "Colicin E2 Release: Lysis, Leakage or Secretion? Possible Role of a Phospholipase," The EMBO J. 3(10):2393-2397.
Suite, et al., (1985), J. Bacteriol. 161:944-948.
Sabik, J. F., Suit, J. L. and Luria, S. E. (1983) J. Bacteriol. 153:1479-1485.
Kobayashi, T. et al., (1986) J. Bacteriol. 166:728-732.
Altieri, M., Suit, J. L., Fan, M.-L. J. and Luria, S.E. (1986) J. Bacteriol. 168:648-654.

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