Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-07-31
2000-02-22
Elliott, George C.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 698, 4352523, 43525232, 4353201, 530350, 5303871, 536 234, 536 237, C07K 1434, C12N 121, C12N 1577, C12P 2102
Patent
active
060279206
ABSTRACT:
A DNA cassette for expression and secretion of a given amino acid, polypeptide or protein in a host strain of corynebacterium, including a sequence which encodes the amino acid, polypeptide or protein, and a region of chromosomal or plasmid DNA, wherein the sequence is situated in the region of chromosomal or plasmid DNA such that the sequence is transcribed with, at the sequence's 5' end, at least one portion of a sequence encoding the signal sequence of the protein PS1 or PS2, the at least one portion ensuring the secretion of the amino acid, polypeptide or protein after translation when the DNA cassette is incorporated into the host strain of corynebacterium.
REFERENCES:
patent: 4965197 (1990-10-01), Liebl et al.
Wilkins Bergey's Manual of Systematic Bacteriology vol. 2 pp. 1261-1434.
M.-A. Petit et al., "Hypersecretion of a cellulase from clostridium thermocellum in bacillus subtilis by induction of chromosomal dna amplification", Bio/Technology, vol. 8, No. 6, Jun. 1990.
PROMEGA product catalogue 1990/91 published by PROMEGA Corporation, Madison, Wisconsin, USA, pp. 10, Fig. 2A, 11, Table 2A, 72-73, and 76-77.
Patent Abstracts of Japan, vol. 13, No. 3 (C-557) (3351) Jan. 6, 1989 & JP-A-63 214 189 (Asahi Chem Ind Co Ltd).
Database WPIL, Derwent Publications Ltd., London, GB; Accession No. 91-310282, DW9142 & US-A-7560035 (Nat Inst of Health), Oct. 09, 1991.
Y. Morinaga et al., "Expression of Escherichia coli promoters in Brevibacterium lactofermentum using the shuttle vector pEB003", J. Biotechnology, vol. 5, No. 4, May 5, 1987, pp. 305-312.
A. Schwarzer et al., "Manipulation of Corynebacterium Glutamicum by Gene Disruption & Replacement", Bio/Technology, vol. 9, No. 1, Jan. 1991, pp. 84-87, Nature America, Inc.
Duchiron Francis
Guyonvarch Armel
Joliff Gwennael
Leblon Gerard
Relano Purification
Elliott George C.
Orsan
Schwartzman Robert
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