System for protease mediated protein expression

Details System for protease mediated protein expression System for protease mediated protein expression

2005-11-17

2008-08-12

Monshipouri, Maryam (Department: 1656)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S252300, C435S320100, C435S069100, C536S023100

Reexamination Certificate

active

07410765

ABSTRACT:
The present invention provides methods and materials that can be used to detect, examine and exploit protease mediated protein expression. An illustrative embodiment of the invention is a polynucleotide encoding a polypeptide having a plurality of operatively linked elements comprising a polypeptide degradation signal that functions as a proteosome substrate; a polypeptide of interest having an observable activity; and a linker disposed between the polypeptide degradation signal and the polypeptide of interest. The linker comprises a protease recognition motif that is capable of being specifically recognized and cleaved by a protease. The activity of the polypeptide of interest is observed when the motif is specifically recognized and cleaved by the protease; and the activity of the polypeptide of interest is decreased or not observed when the motif is not specifically recognized and cleaved by the protease.

REFERENCES:
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