System for producing synthetic promoters

Details System for producing synthetic promoters System for producing synthetic promoters

2006-06-20

2006-06-20

Ketter, James (Department: 1636)

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

C435S320100

Reexamination Certificate

active

07063947

ABSTRACT:
The present application discloses a method for making and selecting a transcription enhancing combined promoter cassette, which includes constructing a library of randomly combined transcription regulatory elements, which comprise double stranded DNA sequence elements that are recognized by transcription regulators; inserting the combined transcription regulatory elements upstream of a minimum promoter followed by a reporter gene in a vector; inserting the vector into a host cell; and then screening for the cells showing enhanced expression of the reporter gene, and identifying the combined promoter cassette in the cell.

REFERENCES:
Li et al., Nature Biotechnology, vol. 17, 1999, pp. 241-245.
Bi et al., Journal of Biotechnology, vol. 93, 2002, pp. 231-242.
Nettelbeck et al., Trends in Genetics, vol. 16, 2000, pp. 174-181.

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