System for producing Enterocytozoon bieneusi live organisms

Details System for producing Enterocytozoon bieneusi live organisms System for producing Enterocytozoon bieneusi live organisms

1997-06-16

1999-04-06

Lankford, Jr., Leon B.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

435260, 435261, 435947, C12N 100, C12N 102, C12N 104, C12N 110

Patent

active

058917071

ABSTRACT:
Described is a system for efficiently producing live Enterocytozoon bieneusi (E. bieneusi) organisms using laboratory animals, preferably Mongolian gerbils. Some important steps are: providing at least one breeding pair; administering to each of such breeding pair a sufficient antibiotic in sufficient dosage to destroy essentially all parasites which might be passed to offspring, especially Trichomonas; permitting such breeding pair to produce and rear offspring as production animals; preventing breeding by the production animals by separating males from females in separate cages; immune-suppressing each production animal sufficiently to permit propagation of live E. bieneusi organisms; infecting each production animal with love E. bieneusi organisms administered in an amount sufficient for such organisms to propagate but insufficient to kill the production animal; and, during a production period following such infecting, collecting the feces of each production animal. The collected fecal material is then prepared for use.

REFERENCES:
patent: 5292725 (1994-03-01), Prendergast
Visvesvara et al. J. Eukaryotic Microbiol. vol. 42(5), pp. 506-510, Abstract enclosed, 1995.
Tzipori et al. J. Infect. Dis. vol. 175 (4), pp. 1016-1020, Abstract enclosed, 1997.

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