System for measuring the efficacy of a sterilization cycle

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving viable micro-organism

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435176, 4352881, 4352887, 422 8208, C12Q 122, C12N 1114, G01N 2164, C12M 140

Patent

active

060635916

ABSTRACT:
A system is used for determining efficacy of a sterilization cycle. A biological sterilization indicator exhibits fluorescence in response to biological activity which is indicative of bacterial growth in the biological sterilization indicator. The biological sterilization indicator is exposed to the sterilization cycle and is placed in a fluorescence reading apparatus. The fluorescence from the biological sterilization indicator is read to obtain a first fluorescence reading. The fluorescence from the biological sterilization indicator is re-read to obtain a second fluorescence reading. The efficacy of the sterilization cycle is determined based on the first and second fluorescence readings.

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