Chemistry: molecular biology and microbiology – Vector – per se
Patent
1995-08-24
2000-01-25
Saunders, David
Chemistry: molecular biology and microbiology
Vector, per se
435 6, 435 72, 435 721, 435328, 435975, 5303873, 5303889, 536 231, C12Q 168, G01N 33566, C12N 510, C07K 1600
Patent
active
060177543
ABSTRACT:
The present invention relates a novel expression system which allows the study of experimental genes of interest on cellular events soon after transfection. The expression system includes a vector which encodes for a recombinant antibody binding unit (rAb). The expression system enables identification and selection of transfected cells from culture to be carried out immediately, within hours, after the transfection
REFERENCES:
patent: 5359046 (1994-10-01), Capon et al.
patent: 5409813 (1995-04-01), Schwartz
patent: 5637481 (1997-06-01), Ledbetter et al.
patent: 5667988 (1997-09-01), Barbas et al.
patent: 5686279 (1997-11-01), Finer et al.
patent: 5731425 (1998-03-01), Brizzard et al.
Clackson, T., et al., "Making Antiboy Fragments Using Phage Display Libraries", Nature, 352:624-628 (1991).
Figini, M., et al., "In Vitro Assembly of Repertoires of Antibody Chains on the Surface of Phage by Renaturation", J. Mol. Biol., 239:68-78 (1994).
Hawkins, R.E., et al., "Selection of Phage Antibodies by Binding Affinity Mimicking Affinity Maturation", J. Mol. Biol., 226:889-896 (1992).
Hoogenboom, H.R., et al., "Building Antibodies from their Genes", Immunol. Rev., 130:41-68 (1992).
Jespers, E.A., et al., "Guiding the Selection of Human Antibodies from Phage Display Repertoires to a Single Epitope of an Antigen", Biotechnology, 12:899-903 (1994).
Larrick, J.W., et al., "Rapid Cloning of Rearranged Immunoglobulin Genes From Human Hybridoma Cells Using Mixed Primers and the Polymerase Chain Reaction", Biochem. Biophys. Res. Comm., 160:1250-1256 (1989).
Marks, J.D., et al., "By-Passing Immunization Human Antibodies from V-Gene Libraries Displayed on Phage", J. Mol. Biol., 222:581-597 (1991).
McCafferty, J., et al., "Phage Antibodies: Filamentous Phage Displaying Antibody Variable Domains", Nature, 348:552-554 (1990).
Morrision, S.L., et al., "Production and Characterization of Genetically Engineered Antibody Molecules", Clin. Chem., 34:1668-1675 (1988).
Orlandi, R., et al., "Cloning Immunoglobulin Variable Domains for Expression by the Polymerase Chain Reaction", Proc. Natl. Acad. Sci., 86:3833-3837 (1989).
Siegall, C.B., et al., "In Vitro and In Vivo Characterization of BR96 sFv-PE40", J. Immunol., 152:2377-2384 (1994).
Winter, G., et al., "Making Antibodies by Phage Display Technology", Annu. Rev. Immunol, 12:433-455 (1994).
Hoogenboom, H.R. et al., "Multi-subunit proteins on the surface of filamentous phage: Methodologies for displaying antibody (Fab) heavy and light chains", Nucleic Acid Res., 19(15):4133-4137 (1991).
Williamson, R.A. et al., "Human monoclonal antibodies against a plethora of viral pathogens from single combinatorial libraries", Proc. Natl. Acad. Sci. USA, 90:4141-4145 (1993).
Chesnut Jonathan D.
Hoeffler James P.
Invitrogen Corporation
Saunders David
VanderVegt F. Pierre
LandOfFree
System for isolating and identifying eukaryotic cells transfecte does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with System for isolating and identifying eukaryotic cells transfecte, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and System for isolating and identifying eukaryotic cells transfecte will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-2315156